CD151 anticorps

N° du produit ABIN118883

L’anticorps Souris Monoclonal anti-CD151 a été validé pour WB, FACS, IHC (p), IP, IHC (fro) et EIA. Il convient pour détecter CD151 dans des échantillons de Humain.

Aperçu rapide pour CD151 anticorps (ABIN118883)

Antigène: CD151

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp CD151 est non-conjugé

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA)

Clone: 11G5a

Détail du produit anti-CD151 anticorps

Specificité: This antibody recognizes the CD151 cell surface antigen, also known as PETA-3.

Purification: Affinity Chromatography on Protein G

Immunogène: Remarks: Spleen cells from immunised BALB/c mice were fused with cells of the X63.Ag8.653 mouse myeloma cell line.

Isotype: IgG1

Information d'application

Indications d'application: ELISA. Flow cytometry: 1/50 - 1/100, use 10 μL of the suggested working dilution to label 10^6 cells or 100 μL of whole blood. Immunohistochemistry on frozen sections. Immunohistochemistry on paraffin sections: 1/100. This product does not require protein digestion pre-treatment but results are enhanced with heat mediated antigen retrieval in 0.01M citrate buffer. Recommended positive control: Tonsil/spleen. Western Blot. Immunoprecipitation.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. Further Comments: It should be noted that CD151 is very closely associated with the alpha3 beta1 integrin in cells and coimmunoprecipitation may occur even in quite stringent conditions .

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL

Buffer: PBS, 0.09 % Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Détails sur CD151

Antigène: CD151

Autre désignation: CD151 / TSPAN24

Sujet: CD151 is expressed by epithelial cells, endothelial cells, platelets, megakaryocytes, monocytes and in the renal glomeruli and proximal and distal tubules. CD151 is not expressed by lymphocytes or granulocytes. More recently CD151 has also been shown to be expressed by erythrocytes, and to carry the MER2 blood group antigen.Synonyms: GP27, Membrane glycoprotein SFA-1, PETA-3, PETA3, Platelet-endothelial tetraspan antigen 3, SFA-1, SFA1, Tetraspanin-24

ID gène: 977

NCBI Accession: NP_001034579

UniProt: P48509