TSH receptor anticorps

N° du produit ABIN118959

Cet anticorps anti-TSH receptor est un anticorps Souris Monoclonal détectant TSH receptor dans WB, FACS, IP, IHC (p) et IHC (fro). Adapté pour Humain.

Aperçu rapide pour TSH receptor anticorps (ABIN118959)

Antigène: TSH receptor (TSHR) (Thyroid Stimulating Hormone Receptor (TSHR))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp TSH receptor est non-conjugé

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: 4C1

Détail du produit anti-TSH receptor anticorps

Specificité: Recognizes the Human thyroid stimulating hormone (TSH) receptor binding to an extracellular domain. The epitope has been mapped to aa 379-384 (2).

Réactivité croisée (Details): Species reactivity (tested):Human.

Purification: Affinity Chromatography on Protein G

Immunogène: External domain of Human TSH receptor Remarks: Spleen cells from immunised BALB/c mice were fused with cells of the NS1/AG4.1 myeloma cell line.

Isotype: IgG2a

Information d'application

Indications d'application: Flow cytometry: use 10 μL of 1/100-1/1,000 diluted antibody to label 10^6 cells in 100 μL. Immunohistochemistry on frozen and paraffin sections (does not require protein digestion pre- treatment nor antigen retrieval using heat treatment prior to staining of paraffin sections). Histology positive control: Thyroid gland. Immunoprecipitation. Western blotting (1/1,000). According to customer information, this antibody has been used successfully on a peptide microarray (2). Not Suitable for ELISA.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL

Buffer: PBS containing 0.09 % Sodium Azide as preservative

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Détails sur TSH receptor

Antigène: TSH receptor (TSHR) (Thyroid Stimulating Hormone Receptor (TSHR))

Autre désignation: Thyrotropin Receptor / TSHR

Sujet: Thyrotropin Receptor, a Glycoprotein Hormone Receptor, plays a central role in controlling thyroid cell metabolism. The TSH receptor can interact with both TSH and autoantibodies against the TSH receptor (thyroid-stimulating immunoglobulins, TSI). Mutations in the receptor are the cause for hyperthyroidism such as gestational hyperthyroidism (hypersensitivity to chorionic gonadotropin) and Grave's disease (autoimmune hyperthyroidism). Multiple isoforms of the thyrotropin receptor are produced by altervative splicing. The thyrotropin receptor has been reported in adipose, adrenal, brain, eye, heart, kidney, skin, thymus, and thyroid. ESTs have been isolated from brain, placenta, and thyroid libraries.Synonyms: LGR3, TSH-R, TSH-receptor, Thyroid-stimulating hormone receptor

ID gène: 7253

NCBI Accession: NP_000360

UniProt: P16473

Pathways: Thyroid Hormone Synthesis