KRT14 anticorps (C-Term) (FITC)
Aperçu rapide pour KRT14 anticorps (C-Term) (FITC) (ABIN118964)
Antigène
Voir toutes KRT14 AnticorpsReactivité
Hôte
Clonalité
Conjugué
Application
Clone
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Épitope
- C-Term
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Specificité
- Recognizes Cytokeratin 14, a type I intermediate filament, expressed by stratifying epithelial cells and can be used to distinguish these cell types from simple epithelial cells, which do not express Cytokeratin 14. Mouse anti Cytokeratin 14, clone LL002 has been reported to be suitable for use in Western blotting (Alam et al. 2011).
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Purification
- Affinity Chromatography on Protein A
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Immunogène
- Last 15 C-terminal residues of Human cytokeratin 14 conjugated to thyroglobulin.
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Isotype
- IgG3
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Indications d'application
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Flow Cytometry: Use 10 μL of neat - 1/10 diluted antibody to label 10^6 cells in 100 μL (Requires Cell Permeabilisation).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
Restrictions
- For Research Use only
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Concentration
- 0.1 mg/mL
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Buffer
- PBS, 0.09 % Sodium Azide, 1 % BSA
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Agent conservateur
- Sodium azide
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Précaution d'utilisation
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Conseil sur la manipulation
- Avoid repeated freezing and thawing.
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Stock
- 4 °C/-20 °C
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Stockage commentaire
- Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
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- KRT14 (Keratin 14 (KRT14))
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Autre désignation
- Cytokeratin 14
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Sujet
- Keratin 14 is a member of the keratin family, the most diverse group of intermediate filaments. It is usually found as a heterotetramer with two molecules of keratin 5, a type II keratin. Together they form the cytoskeleton of epithelial cells. Mutations in the genes for these keratins are associated with epidermolysis bullosa simplex. Keratin 14 has been studied as a prognostic marker in breast cancer.Synonyms: CK14, Cytokeratin-14, K14, KRT14, Keratin 14, Keratin Type I Cytoskeletal 14
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ID gène
- 3861
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NCBI Accession
- NP_000517
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UniProt
- P02533
Antigène
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