Poly-ADP-Ribose anticorps

N° du produit ABIN118982

L’anticorps Souris Monoclonal anti-Poly-ADP-Ribose a été validé pour WB, IHC (p), IHC (fro) et IP. Il convient pour détecter Poly-ADP-Ribose dans des échantillons de Humain.

Aperçu rapide pour Poly-ADP-Ribose anticorps (ABIN118982)

Antigène: Poly-ADP-Ribose (PAR)

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Poly-ADP-Ribose est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunoprecipitation (IP)

Clone: 10H

Détail du produit anti-Poly-ADP-Ribose anticorps

Réactivité croisée (Details): Species reactivity (expected):Broad.
Species reactivity (tested):Human.

Purification: Purified

Immunogène: Purified poly (ADP-ribose) polymer, 10-50 unit chain length.

Isotype: IgG3

Information d'application

Indications d'application: Immunohistology on frozen sections: This product requires protein digestion pre-treatment of paraffin embedded sections e.g. trypsin or pronase prior to staining. Immunoprecipitation. Western Blot: 1/500.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: PBS, 50 % Glycerol

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Détails sur Poly-ADP-Ribose

Antigène: Poly-ADP-Ribose (PAR)

Sujet: PADPR (Poly(ADP-ribose)) is a polymer synthesized by a class of enzymes named poly(ADP-ribose) polymerases (PARP). Using NAD+ as substrate, PARP catalyzes the formation of the polymer PADPR, with chain lengths ranging from 2 to 300 residues, containing approximately 2 % branching in the chain. PADPR becomes attached to nuclear proteins, and to PARP itself (automodification). Under normal conditions, cells display low basal level of PADPR polymer, which can dramatically increase in cells exposed to DNA damaging agents (irradiation, alkylation, etc.). This increase of polymer synthesis is usually transient and is followed by a rapid degradation phase with a short half life which can be less than 1 min. The low endogenous level of polymer in unstimulated cells and its rapid catabolism during DNA damage has been ascribed to high activity of the polymer catabolizing enzyme poly(ADP-ribose) glycohydrolyase (PARG).Synonyms: PADPR