2C11 recognises both native and denatured TSH receptor (binding to an epitope at the carboxy terminus between amino acids 354 and 359). It does inhibit binding of TSH. No cross reactivity has been observed with related LH and FSH receptors. This antibody recognises the mutant TSH receptor known as I167N as well as the wild type molecule.
Purification
Affinity chromatography on Protein G
Immunogène
Recombinant Human TSH receptor. Spleen cells from immunised BALB/c mice were fused with cells of the NS1/Ag4.1 mouse myeloma cell line.
Flow Cytometry. ELISA. Immunoprecipitation: 5-10 μg/mL. Western Blot: 5-10 μg/mL. Functional Assays. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions
For Research Use only
Concentration
1.0 mg/mL
Buffer
PBS, pH 7.2 containing 0.09 % Sodium Azide as preservative
Agent conservateur
Sodium azide
Précaution d'utilisation
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Conseil sur la manipulation
Avoid repeated freezing and thawing.
Stock
4 °C/-20 °C
Stockage commentaire
Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
The thyroid stimulating hormone (TSH) receptor (otherwise known as the thyrotropin receptor) is an important molecule in controlling the growth and function of the normal thyroid.Synonyms: LGR3, TSH-R, TSH-receptor, Thyroid-stimulating hormone receptor