Myeloperoxidase anticorps

N° du produit ABIN119048

Détail du produit anti-Myeloperoxidase anticorps

Antigène: Myeloperoxidase (MPO)

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Myeloperoxidase est non-conjugé

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA)

Purification: Affinity Chromatography on Protein G

Immunogène: Human Myeloperoxidase. Spleen cells from immunised mice were fused with cells of the mouse X63 AG8653 myeloma cell line.

Clone: 2C7

Isotype: IgG1

Information d'application

Indications d'application: Flow Cytometry (1/50-1/100): Membrane permeabilisation is required. The antibody may be of value in the study of myeloid cells and myeloid leukaemias by flow cytometry. Immunhistology on Frozen Sections (1/1000-1/5000). Immunhistology on Paraffin Sections (1/500-1/1000): use bone marrow as positive control. ELISA. Western blotting (Non reducing conditions): ABIN119048 recognises native MPO in Western blots, and the heavy chain following boiling of the sample. The antibody also recognises recombinant MPO in Western blots and weakly in ELISA.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL

Buffer: PBS containing 0.09 % Sodium Azide as preservative

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Références pour anticorps anti-Myeloperoxidase (ABIN119048)

OBrien, Abdulahad, Rutgers, Huitema, OReilly, Coughlan, Harrington, Heeringa, Little, Hickey: "Intermediate monocytes in ANCA vasculitis: increased surface expression of ANCA autoantigens and IL-1β secretion in response to anti-MPO antibodies." dans: Scientific reports, Vol. 5, pp. 11888, (2015) (PubMed).

Schreiber, Xiao, Jennette, Schneider, Luft, Kettritz: "C5a receptor mediates neutrophil activation and ANCA-induced glomerulonephritis." dans: Journal of the American Society of Nephrology : JASN, Vol. 20, Issue 2, pp. 289-98, (2009) (PubMed).

Détails sur Myeloperoxidase

Antigène: Myeloperoxidase (MPO)

Autre désignation: Myeloperoxidase (MPO Produits)

Synonymes: anticorps mKIAA4033, anticorps MPO, anticorps LOC100335032, anticorps POX2', anticorps XPOX2', anticorps mpo, anticorps mpo-A, anticorps pmr-1, anticorps pmr1, anticorps pox2, anticorps xpox2, anticorps myeloperoxidase, anticorps myeloperoxidase L homeolog, anticorps MPO, anticorps Mpo, anticorps LOC100335032, anticorps mpo.L

Sujet: Myeloperoxidase/MPO is an important component of azurophilic granules in neutrophils, being involved in microbicidal processes. The protein is a multimer of 2 heavy chains (55 kD) and two light chains (15 kD), the heavy chains being linked by a disulphide bond. Myeloperoxidase is a hemoprotein that is abundantly expressed in neutrophils and secreted during their activation. Native Myeloperoxidase is represented as a covalently bound tetrameric complex of two glycosylated alpha chains (MW 59-64 kDa) and two unglycosylated beta chains (MW 14 kDa) with total MW 150 kDa and theoretical pI 9.2. Traditionally Myeloperoxidase was considered as a main target of anti-neutrophil cytoplasm antibodies (ANCA), the serological markers for certain systemic vasculities e.g. periarteriitis nodosa, microscopic polyarteriitis and pulmonary eosinophilic granulomatosis (Churg-Strauss syndrome). Low to moderate anti-Myeloperoxidase auto-antibody levels are also reported in rheumatoid arthritis. Recently it was shown that Myeloperoxidase participates in the initiation and progression of cardiovascular disease. It possesses potent proinflammatory properties and may contribute directly to tissue injury. Now Myeloperoxidase is under consideration as one of the most promising cardiac markers.Synonyms: MPO

ID gène: 9606

UniProt: P05164

Pathways: Chromatin Binding