Huntingtin anticorps (AA 2703-2911)

N° du produit ABIN119307

Cet anticorps anti-Huntingtin est un anticorps Souris Monoclonal détectant Huntingtin dans WB, IP et IHC (fro). Adapté pour Humain, Souris et Lapin.

Aperçu rapide pour Huntingtin anticorps (AA 2703-2911) (ABIN119307)

Antigène: Huntingtin (HTT)

Reactivité: Humain, Souris, Lapin

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Huntingtin est non-conjugé

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: HDC8A4

Détail du produit anti-Huntingtin anticorps

Épitope: AA 2703-2911

Specificité: This antibody reacts with an epitope corresponding to the HDC region (2703 - 2911 amino acids) of the huntingtin protein. Clone HDC8A4 detects a 350KD band on western blots but also detects smaller degradation products of huntingtin. Clone HDC8A4 recognises both denatured and native huntingtin in human brain. The combined use of clone HDC8A4 (SM1662), HDB4E10 (SM1661) and HDA3E10 (SM1660) demonstrate that huntingtin is enriched in neuronal cells in the brain.

Purification: Affinity chromatography on Protein G

Immunogène: Recombinant protein corresponding to amino acids 2703 - 2911 of huntingtin

Isotype: IgG1

Information d'application

Indications d'application: Immunohistochemistry on frozen sections. Immunoprecipitation. Western Blot.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL

Buffer: PBS containing 0.09 % Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Détails sur Huntingtin

Antigène: Huntingtin (HTT)

Autre désignation: Huntingtin

Sujet: Huntington's disease (HD) is a neurodegenerative disorder caused by an expanding polyglutamine repeat in the huntingtin gene. HD is a mid-life onset autosomal dominant neurodegeneative disease that is characterized by psychiatric disorders, dementia, and involuntary movements (chorea), leading to death in 10-20 years. The HD gene product is widely expressed in human tissues, with the highest level of expression in the brain. The huntingtin gene product is expressed at similar levels in patients and controls, which suggests that the expansion of the polyglutamine repeat induces a toxic gain of function perhaps through interactions with other cellular proteins. Using yeast two-hybrid system, HAP1 (huntingtin associated protein 1) has been identified, that associates with huntingtin protein. The In vitro data suggest that the association between HAP1 and huntingtin is enhanced by increasing length of glutamine repeat.Synonyms: HD, HTT, Huntington Disease Protein, IT15

ID gène: 3064

NCBI Accession: NP_002102

UniProt: P42858

Pathways: Signalisation PI3K-Akt, Hormone Transport, Transition Metal Ion Homeostasis, Tube Formation, Protein targeting to Nucleus, Dicarboxylic Acid Transport