His Tag anticorps

N° du produit ABIN119353

Cet anticorps Souris Monoclonal détecte spécifiquement His Tag dans WB, IP, IHC (fro) et EIA.

Aperçu rapide pour His Tag anticorps (ABIN119353)

Antigène: His Tag

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp His Tag est non-conjugé

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA)

Clone: AD1-1-10

Détail du produit anti-His Tag anticorps

Séquence: HHHHHH

Purification: Affinity Chromatography on Protein G

Immunogène: PAX6 transcription factor linked to histidine tag. Remarks: Spleen cells from immunised Balb/c mice were fused with cells of the mouse NS1 myeloma cell line.

Isotype: IgG1

Information d'application

Indications d'application: Immunhistology on Frozen Sections. ELISA. Immunoprecipitation. Western Blot (1/500-1/1000). In Western blotting of bacterial extracts the antibody has been shown not to cross-react with any endogenous products, although some cross-reactivity may be seen with extracts of insect or mammalian cells.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL

Buffer: PBS containing 0.09 % Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Détails sur His Tag

Antigène: His Tag

Autre désignation: 6xHistidine Epitope Tag

Classe de substances: Tag

Sujet: The H-H-H-H-H-H motif is used as a tag on many recombinant proteins to facilitate purification.