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CD43 anticorps

Cet anticorps Souris Monoclonal détecte spécifiquement CD43 dans FACS, WB, IHC (p), IP et IHC (fro). Il présente une réactivité envers Humain.
N° du produit ABIN119530

Aperçu rapide pour CD43 anticorps (ABIN119530)

Antigène

Voir toutes CD43 (SPN) Anticorps
CD43 (SPN) (Sialophorin (SPN))

Reactivité

  • 154
  • 36
  • 18
  • 1
  • 1
Humain

Hôte

  • 92
  • 84
  • 27
Souris

Clonalité

  • 133
  • 66
  • 4
Monoclonal

Conjugué

  • 86
  • 18
  • 18
  • 17
  • 11
  • 6
  • 4
  • 4
  • 3
  • 3
  • 3
  • 3
  • 3
  • 3
  • 3
  • 3
  • 2
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
Cet anticorp CD43 est non-conjugé

Application

  • 129
  • 116
  • 82
  • 55
  • 44
  • 30
  • 26
  • 13
  • 7
  • 6
  • 6
  • 5
  • 5
  • 4
  • 3
  • 2
  • 1
  • 1
  • 1
  • 1
Flow Cytometry (FACS), Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone

DFT-1
  • Specificité

    This antibody recognises the CD43 cell surface antigen also known as leukosialin and sialophorin.

    Purification

    Affinity Chromatography on Protein G

    Isotype

    IgG1
  • Indications d'application

    Flow cytometry: 1/100, use 10 μL of the suggested working dilution to label 10^6 cells. Immunohistochemistry on frozen sections. Immunohistochemistry on paraffin sections: 1/10 - 1/100, This product does not require protein digestion pre-treatment of paraffin sections however, staining of paraffin embedded formalin fixed tissues may be improved in some cases by enzyme pretreatment using Trypsin. Recommended positive control tissue: Tonsil.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.

    Restrictions

    For Research Use only
  • Buffer

    PBS, pH 7.4, 0.09 % Sodium Azide

    Agent conservateur

    Sodium azide

    Précaution d'utilisation

    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Conseil sur la manipulation

    Avoid repeated freezing and thawing.

    Stock

    4 °C/-20 °C

    Stockage commentaire

    Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
  • Antigène

    CD43 (SPN) (Sialophorin (SPN))

    Autre désignation

    CD43 / Leukosialin

    Sujet

    CD43 is a heavily glycosylated and sialylated single chain glycoprotein, expressed by all leucocytes with the exception of most B lymphocytes.Synonyms: Galactoglycoprotein, Leukocyte sialoglycoprotein, SPN, Sialophorin

    ID gène

    6693

    NCBI Accession

    NP_001025459

    UniProt

    P16150

    Pathways

    Regulation of Leukocyte Mediated Immunity
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