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CD177 anticorps

L’anticorps Souris Monoclonal anti-CD177 a été validé pour WB, FACS et IP. Il convient pour détecter CD177 dans des échantillons de Humain et Macaque rhésus.
N° du produit ABIN119765

Aperçu rapide pour CD177 anticorps (ABIN119765)

Antigène

Voir toutes CD177 Anticorps
CD177 (CD177 Molecule (CD177))

Reactivité

  • 51
  • 7
  • 5
  • 4
  • 4
  • 3
  • 2
Humain, Macaque rhésus

Hôte

  • 32
  • 23
  • 1
Souris

Clonalité

  • 35
  • 21
Monoclonal

Conjugué

  • 27
  • 7
  • 6
  • 5
  • 4
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
Cet anticorp CD177 est non-conjugé

Application

  • 31
  • 22
  • 13
  • 13
  • 11
  • 5
  • 5
  • 3
  • 2
  • 1
  • 1
Western Blotting (WB), Flow Cytometry (FACS), Immunoprecipitation (IP)

Clone

MEM-166
  • Specificité

    This antibody recognizes CD177 (Neutrophil Glycoprotein NB1). Antibodies against NB1 can be involved in neonatal alloimmune neutropenia (NAN). MEM-166 recognizes CD177 under Non-Reducing Conditions.

    Purification

    Affinity Chromatography on Protein G

    Isotype

    IgG1
  • Indications d'application

    Flow cytometry (1/50 - 1/100): use 10 μL of the suggested working dilution to label 10^6 cells in 100 μL. Western blot: Non-reducing conditions. Immunoprecipitation.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.

    Restrictions

    For Research Use only
  • Concentration

    1.0 mg/mL

    Buffer

    PBS, 0.09 % Sodium Azide

    Agent conservateur

    Sodium azide

    Précaution d'utilisation

    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Conseil sur la manipulation

    Avoid repeated freezing and thawing.

    Stock

    4 °C/-20 °C

    Stockage commentaire

    Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
  • Antigène

    CD177 (CD177 Molecule (CD177))

    Autre désignation

    CD177

    Sujet

    CD177 (NP1), a glycosyl phosphatidylinositol (GPI) linked N glycosylated cell surface glycoprotein, was first described in a case of neonatal alloimmune neutropenia and analyses showed cell surface expression of a 60 kDa protein, 14 kDa greater than the predicted size, probably due to the presence of 3 potential N glycosylation sites. Immunohistochemistry demonstrated expression in bone marrow early erythroblasts, megakaryocytes, promyelocytes, and myelocytes. The neutrophil NB1 antigen (CD177) is expressed by 97 % of the caucasian population.Synonyms: HNA-2a, HNA2a, Human neutrophil alloantigen 2a, NB1, NB1 glycoprotein, PRV1, Polycythemia rubra vera protein 1

    ID gène

    57126

    NCBI Accession

    NP_065139

    UniProt

    Q8N6Q3
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