CD163 anticorps

N° du produit ABIN120083

L’anticorps Souris Monoclonal anti-CD163 a été validé pour WB, FACS, IF, IHC (p), IHC (fro) et EIA. Il convient pour détecter CD163 dans des échantillons de Humain, Singe et Mouton.

Aperçu rapide pour CD163 anticorps (ABIN120083)

Antigène: CD163

Reactivité: Humain, Singe, Mouton

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp CD163 est non-conjugé

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA)

Clone: EDHu-1

Détail du produit anti-CD163 anticorps

Specificité: This antibody recognises the CD163 cell surface antigen. Clone EDHu-1 is reported to inhibit the binding of haptoglobin:haemoglobin to CD163.

Réactivité croisée (Details): Species reactivity (tested):Human, Rhesus Monkey, Sheep.

Purification: Affinity Chromatography on Protein A

Immunogène: Leucocytes harvested from pleural cavity of patients with idiopathic spontaneous pneumothorax.

Isotype: IgG1

Information d'application

Indications d'application: Flow Cytometry. Immunohistochemistry on frozen sections. Western Blot.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL

Buffer: PBS, pH 7.4, 0.09 % Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Détails sur CD163

Antigène: CD163

Autre désignation: CD163

Sujet: CD163 is a 130-140kD glycoprotein expressed by tissue macrophages. CD163 is not expressed by resting peripheral blood leucocytes, but expression may be induced on monocytes by culture in dexamethasone. It is a member of the scavenger receptor cysteine-rich superfamily and is a receptor for the hemoglobin-haptoglobin complex. CD163 is expressed exclusively on the cell surface of human monocytes and macrophages that evolve predominantly in the late phase of inflammation. CD163 is present on all CD14 positive monocytes, most CD64 positive monocytes, and shows higher expression on CD16 positive monocytes. CD163 is upregulated on mononuclear phagocytes by IL-10, IL-6 and dexamethasone. Lipopolysaccharide (LPS) and phorbol myristate acetate (PMA) both induce shedding of CD163 from the cell surface into plasma or cell supernatant.Synonyms: Hemoglobin scavenger receptor, M130, Macrophage marker, Scavenger receptor cysteine-rich type 1 protein M130

ID gène: 9332

NCBI Accession: NP_004235

UniProt: Q86VB7