TUBA1B anticorps
-
- Antigène Voir toutes TUBA1B Anticorps
- TUBA1B (Tubulin, alpha 1B (TUBA1B))
-
Reactivité
- Avian, Mammifères, Saccharomyces cerevisiae
-
Hôte
- Rat
-
Clonalité
- Monoclonal
-
Conjugué
- Cet anticorp TUBA1B est non-conjugé
-
Application
- Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA), Immunoprecipitation (IP), Radioimmunoassay (RIA)
- Specificité
- SM2202P recognises the alpha subunit of Tubulin, specifically binding tyrosylated Tubulin (Tyr-Tubulin) (2). The epitope recognised by this antibody has been extensively studied and would appear to be a linear sequence requiring an aromatic residue at the C terminus, with the two adjacent amino acids being negatively charged (represented by Gly-Gly-Tyr in Tyr-Tubulin). The antibody has been used in epitope tagging procedures to detect proteins tagged with a C-terminal Gly-Gly-Phe epitope. These sequence requirements have been reported to result in some cross-reactivity with other proteins in certain circumstances, including E. coli rec A and oxidized Actin (5).
- Purification
- Affinity Chromatography on Protein G
- Immunogène
- Yeast Tubulin. Spleen cells from immunised LOU rats were fused with cells of the Y3.Ag.1.2.3 rat myeloma cell line.
- Clone
- YL1-2
- Isotype
- IgG2a
- Top Product
- Discover our top product TUBA1B Anticorps primaire
-
-
- Indications d'application
-
Immunohistochemistry on frozen sections. ELISA (1/100-1/1000, routinely tested in ELISA on Tubulin) (3). Immunoprecipitation. Radioimmunoassay. Clone YL1/2 has also been reported to work in western blotting (3).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - Restrictions
- For Research Use only
-
- Concentration
- 1.0 mg/mL
- Buffer
- Phosphate buffered saline pH 7.4, 0.09 % Sodium Azide
- Agent conservateur
- Sodium azide
- Précaution d'utilisation
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Conseil sur la manipulation
- Avoid repeated freezing and thawing.
- Stock
- 4 °C/-20 °C
- Stockage commentaire
- Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
-
- Antigène
- TUBA1B (Tubulin, alpha 1B (TUBA1B))
- Autre désignation
- alpha Tubulin / TUBA1B (TUBA1B Produits)
- Synonymes
- anticorps RGD1565476, anticorps K-ALPHA-1, anticorps cb944, anticorps fb22g06, anticorps tuba1, anticorps wu:fb22g06, anticorps Tuba2, anticorps TUBA3, anticorps k-alpha-1, anticorps tuba, anticorps Calpha1, anticorps Tuba1, anticorps TUBA1B, anticorps tubulin, alpha 1B, anticorps tubulin alpha 1b, anticorps tubulin, alpha 1b, anticorps tubulin alpha 1b L homeolog, anticorps tubulin alpha-1B chain, anticorps Tubulin alpha-1B chain, anticorps Tuba1b, anticorps TUBA1B, anticorps tuba1b, anticorps tuba1b.L, anticorps LOC100445003, anticorps LOC102178700
- Sujet
- Tubulin is the major building block of microtubules. This intracellular cylindrical filamentous structure is present in almost all eukaryotic cells. Microtubules function as structural and mobile elements in mitosis, intracellular transport, flagellar movement and in the cytoskeleton. Tubulin is a heterodimer which consists of alpha tubulin and beta tubulin.Synonyms: Alpha-tubulin ubiquitous, Tubulin K-alpha-1, Tubulin alpha-1B chain, Tubulin alpha-ubiquitous chain
- ID gène
- 10376
- NCBI Accession
- NP_006073
- UniProt
- P68363
- Pathways
- Dynamique des Microtubules, M Phase
-