TUBA1B anticorps

N° du produit ABIN120144

Cet anticorps anti-TUBA1B Monoclonal Rat (Clone YL1-2) (ABIN120144) détecte spécifiquement TUBA1B dans WB, IF, IHC (fro), IP, EIA et RIA. L’anticorps est réactif avec des échantillons de Avian, Mammifères et Saccharomyces cerevisiae.

Aperçu rapide pour TUBA1B anticorps (ABIN120144)

Antigène: TUBA1B (Tubulin, alpha 1B (TUBA1B))

Reactivité: Avian, Mammifères, Saccharomyces cerevisiae

Hôte: Rat

Clonalité: Monoclonal

Conjugué: Cet anticorp TUBA1B est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunoprecipitation (IP), Enzyme Immunoassay (EIA), Radioimmunoassay (RIA)

Clone: YL1-2

Détail du produit anti-TUBA1B anticorps

Specificité: SM2202P recognises the alpha subunit of Tubulin, specifically binding tyrosylated Tubulin (Tyr-Tubulin) (2). The epitope recognised by this antibody has been extensively studied and would appear to be a linear sequence requiring an aromatic residue at the C terminus, with the two adjacent amino acids being negatively charged (represented by Gly-Gly-Tyr in Tyr-Tubulin). The antibody has been used in epitope tagging procedures to detect proteins tagged with a C-terminal Gly-Gly-Phe epitope. These sequence requirements have been reported to result in some cross-reactivity with other proteins in certain circumstances, including E. coli rec A and oxidized Actin (5).

Purification: Affinity Chromatography on Protein G

Immunogène: Yeast Tubulin. Spleen cells from immunised LOU rats were fused with cells of the Y3.Ag.1.2.3 rat myeloma cell line.

Isotype: IgG2a

Information d'application

Indications d'application: Immunohistochemistry on frozen sections. ELISA (1/100-1/1000, routinely tested in ELISA on Tubulin) (3). Immunoprecipitation. Radioimmunoassay. Clone YL1/2 has also been reported to work in western blotting (3).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL

Buffer: Phosphate buffered saline pH 7.4, 0.09 % Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Détails sur TUBA1B

Antigène: TUBA1B (Tubulin, alpha 1B (TUBA1B))

Autre désignation: alpha Tubulin / TUBA1B

Sujet: Tubulin is the major building block of microtubules. This intracellular cylindrical filamentous structure is present in almost all eukaryotic cells. Microtubules function as structural and mobile elements in mitosis, intracellular transport, flagellar movement and in the cytoskeleton. Tubulin is a heterodimer which consists of alpha tubulin and beta tubulin.Synonyms: Alpha-tubulin ubiquitous, Tubulin K-alpha-1, Tubulin alpha-1B chain, Tubulin alpha-ubiquitous chain

ID gène: 10376

NCBI Accession: NP_006073

UniProt: P68363

Pathways: Dynamique des Microtubules, M Phase