MADCAM1 anticorps (Biotin)

N° du produit ABIN120235

Cet anticorps Souris Monoclonal détecte spécifiquement MADCAM1 dans FACS et EIA. Il présente une réactivité envers Humain.

Aperçu rapide pour MADCAM1 anticorps (Biotin) (ABIN120235)

Antigène: MADCAM1 (Mucosal Vascular Addressin Cell Adhesion Molecule 1 (MADCAM1))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp MADCAM1 est conjugé à/à la Biotin

Application: Flow Cytometry (FACS), Enzyme Immunoassay (EIA)

Clone: 314G8

Détail du produit anti-MADCAM1 anticorps (Biotin)

Specificité: This antibody recognises mucosal addressin cell adhesion molecule 1 (MAdCAM-1). Clone 314G8 does not recognise MAdCAM-1 in the gut lamina propria. Clone 314G8 reacts with the ligand-binding first Ig domain and reports suggest that a splice variant exists in the gut which is not recognised by clone 314G8. Clone 314G8 is reported to block the interaction of MAdCAM-1 with alpha 4 beta 7. We recommend the use of SM2237LE for this purpose.

Purification: Affinity Chromatography on Protein G.

Immunogène: Recombinant soluble MAdCAM-1-fc fusion protein. Spleen cells from immunised Balb/c mice were fused with cells of the mouse P3X63Ag8.653 myeloma cell line.

Isotype: IgG1

Information d'application

Indications d'application: Flow Cytometry: (Neat-1/10): Use 10 μL of the suggested working dilution to label 10^6 cells in 100 μL. ELISA: 1-10 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 0.1 mg/mL

Buffer: PBS, pH 7.4 containing 0.09 % Sodium Azide and 1 % Bovine Serum Albumin.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Détails sur MADCAM1

Antigène: MADCAM1 (Mucosal Vascular Addressin Cell Adhesion Molecule 1 (MADCAM1))

Autre désignation: MAdCAM1

Sujet: MAdCAM-1 is a 60kD cell surface protein that is involved in lymphocyte trafficking. MAdCAM-1 is expressed on high endothelial venules of Peyers patches and mesenteric lymph nodes. MAdCAM-1 expression has also been reported in the gut lamina propria.Synonyms: MAdCAM-1, Mucosal addressin cell adhesion molecule 1, hMAdCAM-1

ID gène: 8174

NCBI Accession: NP_570116

UniProt: Q13477