Factor VIII anticorps

N° du produit ABIN120341

L’anticorps anti-Factor VIII Monoclonal Souris est utilisé pour la détection de Factor VIII dans des échantillons de Humain et Porc. Il a été validé pour WB, EIA et RIA.

Aperçu rapide pour Factor VIII anticorps (ABIN120341)

Antigène: Factor VIII (F8) (Coagulation Factor VIII (F8))

Reactivité: Humain, Porc

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Factor VIII est non-conjugé

Application: Western Blotting (WB), Enzyme Immunoassay (EIA), Radioimmunoassay (RIA)

Clone: RFFVIIIC-8

Détail du produit anti-Factor VIII anticorps

Specificité: Clone RFFVIIIC/8 is a very potent coagulation inhibitor. This antibody recognizes an epitope towards the N-terminus of full length Factor VIII. It also recognises the 210 kDa, 90 kDa and 40 kDa cleavage products. This clone does not cross-react with von Willebrand factor.

Aucune reactivité croisée: Souris, Chien, Rat (Rattus)

Réactivité croisée (Details): Species reactivity (tested):Human and Pig.

Purification: Affinity Chromatography on Protein G from tissue culture supernatant

Immunogène: Affinity purified Human Factor VIII

Isotype: IgG1

Information d'application

Indications d'application: ELISA: 1:200 - 1:2000. Western blotting: 1:20 - 1:200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL

Buffer: PBS, 0.09 % Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Détails sur Factor VIII

Antigène: Factor VIII (F8) (Coagulation Factor VIII (F8))

Sujet: Human Factor VIII is an essential blood coagulation factor. Whilst circulating in the blood, it is mostly stably complexed to von Willebrand factor. It is activated through cleavage at various sites, dissociates from the complex and interacts with Factor IXa, in the presence of calcium ions and phospholipids, to convert Factor X to the activated Factor Xa, which activates thrombin. Thrombin cleaves fibrinogen into fibrin, which polymerises and crosslinks to form a blood clot. The activated Factor VIII is proteolytically inactivated and cleared from the bloodstream. Defects in Factor VIII cause haemophilia A, a disorder characterised by the body's inability to control blood clotting. This could result in severe blood loss, even with minor injuries.Synonyms: AHF, Antihemophilic factor, F8C, Procoagulant component

ID gène: 2157

NCBI Accession: NP_000123

UniProt: P00451