Immunocytochemistry. Western blot. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions
For Research Use only
Concentration
1.0 mg/mL
Buffer
PBS, pH ~7.4, 15 mM Sodium Azide
Agent conservateur
Sodium azide
Précaution d'utilisation
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Stock
4 °C/-20 °C
Stockage commentaire
Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing. Shelf Life: one year from despatch.
Date de péremption
12 months
Tahirovic, Hellal, Neukirchen, Hindges, Garvalov, Flynn, Stradal, Chrostek-Grashoff, Brakebusch, Bradke: "Rac1 regulates neuronal polarization through the WAVE complex." dans: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 30, Issue 20, pp. 6930-43, (2010) (PubMed).
Dráber, Slavícková, Sládecek, Viklický: "Monoclonal antibodies to Escherichia coli beta-galactosidase and their use for detection and purification of recombinant expression products." dans: Hybridoma, Vol. 11, Issue 3, pp. 385-90, (1992) (PubMed).
Bazil, Horejsí, Baudys, Kristofová, Strominger, Kostka, Hilgert: "Biochemical characterization of a soluble form of the 53-kDa monocyte surface antigen." dans: European journal of immunology, Vol. 16, Issue 12, pp. 1583-9, (1987) (PubMed).
Beta galactosidase is coded by a gene (lac z) in the lac operon of Escherichia coli. It is a metalloenzyme that splits lactose into glucose and galactose. It hydrolyzes terminal, non-reducing beta-D-galactose residues in beta-D-galactosides. Activation by cations seems to be substrate dependent. K+, Na+, NH4+, Rb+, Cs+ and Mn++ all activate enzyme activity based upon the substrate used.Synonyms: Beta-Gal Fusion Protein, Beta-Gal tag, JW0335, Lactase, b0344, lacZ tag