Cytokeratin 7 anticorps

N° du produit ABIN120627

Cet anticorps anti-Cytokeratin 7 Monoclonal Souris (Clone C-46) (ABIN120627) détecte spécifiquement Cytokeratin 7 dans WB, IF, IHC (p), IP et IHC (fro). L’anticorps est réactif avec des échantillons de Humain et Boeuf (Vache).

Aperçu rapide pour Cytokeratin 7 anticorps (ABIN120627)

Antigène: Cytokeratin 7 (KRT7) (Keratin 7 (KRT7))

Reactivité: Humain, Boeuf (Vache)

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Cytokeratin 7 est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: C-46

Détail du produit anti-Cytokeratin 7 anticorps

Specificité: The antibody C-46 reacts with Cytokeratin peptides 7 and 17 (54 and 46 kDa).

Réactivité croisée (Details): Species reactivity (tested):Human, Porcine, Bovine.
Also reacts to: KRT17

Attributs du produit: Synonyms: KRT7, KRT-7, SCL, CK-7, CK7, Keratin-7, K7, K-7, Sarcolectin, Keratin type II cytoskeletal 7

Purification: Protein A-affinity chromatography

Pureté: > 95 % by SDS-PAGE

Immunogène: Cytoskeleton preparation of HeLa human cervix carcinoma cell line.

Isotype: IgG1

Information d'application

Indications d'application: Immunoprecipitation. Immunocytochemistry. Western Blot: 2-3 mg/mL, 60 min in RT, Boil for 3 min, Resuspend dry pelet in solubilizelysis buffer (with carbamide and detergent NP-40), Mix lysate with reducing SDS-PAGE (+merkaptoethanol) sample buffer, Reducing conditions. Positive control: HeLa human cell line. Immunohistochemistry on paraffin sections: 20 mg/mL, 0,1 % pepsin, 30 min, 37C, 4Covernight, chromogen DAB. Positive tissue: pancreas.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL

Buffer: PBS, pH ~7.4, 15 mM Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C/-20 °C

Stockage commentaire: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.

Date de péremption: 12 months

Détails sur Cytokeratin 7

Antigène: Cytokeratin 7 (KRT7) (Keratin 7 (KRT7))

Autre désignation: Cytokeratin 7

Sujet: Cytokeratins are a member of intermediate filaments subfamily represented in epithelial tissues. Cytokeratins are a subfamily of intermediate filaments and characterized by remarkable biochemical diversity. Cytokeratins are represented in epithelial tissues by at least 20 different polypeptides, molecular weight between 40 kDa and 68 kDa. The individual cytokeratin polypeptides are designated 1 to 20 and divided into the type I (acidic cytokeratins 9-20) and type II (basic to neutral cytokeratins 1-8) families.Synonyms: CK-7, CK7, K-7, K7, KRT-7, KRT7, Keratin type II cytoskeletal 7, Keratin-7, SCL, Sarcolectin

ID gène: 3855

UniProt: P08729