CD38 anticorps

N° du produit ABIN120671

Il existe 1 publication pour ce produit. L’anticorps anti-CD38 Monoclonal Souris est utilisé pour la détection de CD38 dans des échantillons de Humain. Il a été validé pour FACS, WB, IHC (p) et IHC (fro).

Aperçu rapide pour CD38 anticorps (ABIN120671)

Antigène: CD38

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp CD38 est non-conjugé

Application: Flow Cytometry (FACS), Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: HIT2

Détail du produit anti-CD38 anticorps

Specificité: The antibody reacts with CD38 (T10), a 45 kDa type II transmembrane glycoprotein strongly expressed mainly on plasma cells and activated T and B lymphocytes, it is an antigenic marker of lymphoid cells.

Purification: Protein-G Affinity Chromatography.

Immunogène: Peripheral blood mononuclear cells of a patient suffering with CLL

Isotype: IgG1

Information d'application

Indications d'application: Flow Cytometry. Western Blotting (non reducing conditions): 2 mg/mL. Positive control: RAJI human cell line. Sample preparation: Resuspend approx. 50 mil. cells in 1 mLcold Lysis buffer (1 % laurylmaltoside in 20 mM Tris/Cl, 100 mM NaCl pH 8.2, 50 mM NaF including Protease inhibitor Cocktail). Incubate 60 min on ice. Centrifuge to remove cell debris. Mix lysate with non- reducing SDS-PAGE sample buffer. Boil for 5 min. Immunohistochemistry on frozen sections.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL

Buffer: PBS, pH ~7.4, 15 mM Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C/-20 °C

Stockage commentaire: Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf Life: one year from despatch.

Date de péremption: 12 months

Références pour anticorps anti-CD38 (ABIN120671)

Qu, Shen, Shen, Hu, Yang, Wang, Yan, Zhang: "Open-tubular gas chromatography using capillary coated with octadecylamine-capped gold nanoparticles." dans: Analytica chimica acta, Vol. 609, Issue 1, pp. 76-81, (2008) (PubMed).

Détails sur CD38

Antigène: CD38

Sujet: CD38 (NAD+ glycohydrolase) is a type II transmembrane glycoprotein able to induce activation, proliferation and differentiation of mature lymphocytes and mediate apoptosis of myeloid and lymphoid progenitor cells. Another role of CD38 is provided by enzymatic activity of its extracellular part. CD38 acts as NAD+ glycohydrolase converting NAD+ into ADP-ribose, as ADP-ribosyl cyclase producing cADPR and as cADPR hydrolase, thus affecting levels of calcium-mobilizing metabolites. ADPR produced by CD38 serves as an important second messenger of neutrophil and dendritic cell migration.Synonyms: ADP-ribosyl cyclase 1, Cyclic ADP-ribose hydrolase 1, T10, cADPr hydrolase 1

ID gène: 952

UniProt: P28907