alpha Fetoprotein anticorps

N° du produit ABIN120681

Cet anticorps anti-alpha Fetoprotein Monoclonal Souris (Clone AFP-01) (ABIN120681) détecte spécifiquement alpha Fetoprotein dans WB, IP et EIA. L’anticorps est réactif avec des échantillons de Humain.

Aperçu rapide pour alpha Fetoprotein anticorps (ABIN120681)

Antigène: alpha Fetoprotein (AFP) (alpha-Fetoprotein (AFP))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp alpha Fetoprotein est non-conjugé

Application: Western Blotting (WB), Immunoprecipitation (IP), Enzyme Immunoassay (EIA)

Clone: AFP-01

Détail du produit anti-alpha Fetoprotein anticorps

Specificité: The antibody reacts with 70 kDa alpha-Fetoprotein (AFP).

Réactivité croisée (Details): Species reactivity (tested):Human.

Purification: Precipitation Methods

Immunogène: Purified alpha-Fetoprotein

Isotype: IgG1

Information d'application

Indications d'application: Immunoprecipitation: Interaction of the antibody with AFP is dependent on the presence of calcium ions (strongly inhibited by chelating agents). Such characteristics of the antibody can be exploited for immunoaffinity purification of APF under mild elution conditions. ELISA: The antibody has been tested as the detection antibody in a sandwich ELISA for analysis of human alpha-Fetoprotein in combination with clone AFP-11 (cat. no. ABIN120683). Western Blotting: Positive material: Hep G2 human hepatocellular carcinoma cell line. Sample preparation: Resuspend approx. 50 mil. cells in 1 mLcold Lysis buffer (1 % laurylmaltoside in 20 mM Tris/Cl, 100 mM NaCl pH 8.2, 50 mM NaF including Protease inhibitor Cocktail). Incubate 60 min on ice. Centrifuge to remove cell debris. Mix lysate with non- reducing/reducing Laemmli SDS-PAGE sample buffer. Application note: Both non-reducing and reducing conditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH ~7.4 with 15 mM Sodium Azide as preservative

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C/-20 °C

Stockage commentaire: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf Life: one year from despatch.

Date de péremption: 12 months

Détails sur alpha Fetoprotein

Antigène: alpha Fetoprotein (AFP) (alpha-Fetoprotein (AFP))

Sujet: AFP is an oncofetal antigen. AFP is a major fetal plasma protein, but is not present in healthy adult tissues. Elevated AFP concentrations in adult plasma may be an early marker of hepatocellular carcinoma or teratoblastoma, while high concentrations in amniotic fluid may indicate severe congenital defects of a fetus.Synonyms: Alpha-1-fetoprotein, Alpha-fetoglobulin, HPAFP

ID gène: 174

UniProt: P02771

Pathways: C21-Steroid Hormone Metabolic Process