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Blood Group Lewis B anticorps

L’anticorps Souris Monoclonal anti-Blood Group Lewis B a été validé pour IHC (p), WB et EIA. Il convient pour détecter Blood Group Lewis B dans des échantillons de Humain.
N° du produit ABIN120686

Aperçu rapide pour Blood Group Lewis B anticorps (ABIN120686)

Antigène

Blood Group Lewis B

Reactivité

  • 14
  • 12
  • 10
Humain

Hôte

  • 14
Souris

Clonalité

  • 14
Monoclonal

Conjugué

  • 6
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
Cet anticorp Blood Group Lewis B est non-conjugé

Application

  • 12
  • 9
  • 9
  • 4
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB), Enzyme Immunoassay (EIA)

Clone

2-25LE
  • Réactivité croisée (Details)

    Species reactivity (tested):Human.

    Purification

    Affinity Chromatography on Protein A.

    Immunogène

    Mucin isolated by size exclusion chromatography from an ovarian cyst fluid (pure endocervical type according to the Fenoglio's classification)

    Isotype

    IgG1
  • Indications d'application

    Immunohistochemistry: 2-4 μg/mL for 30 min at RT. Staining of formalin-fixed tissues REQUIRES boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min. followed by cooling at RT for 20 min. Use human colon CA for postive control. Western blot. ELISA.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.

    Restrictions

    For Research Use only
  • Concentration

    1.0 mg/mL

    Buffer

    PBS, pH 7.4, with 15 mM Sodium Azide as preservative.

    Agent conservateur

    Sodium azide

    Précaution d'utilisation

    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Stock

    4 °C/-20 °C

    Stockage commentaire

    Store at 2-8 °C for 6 months. For long-term storage aliquot and store at -20 °C. Avoid freeze/thaw cycles.
    Shelf Life: one year from despatch.

    Date de péremption

    12 months
  • Antigène

    Blood Group Lewis B

    Sujet

    Blood group antigens are generally defined as molecules formed by sequential addition of saccharides to the carbohydrate side chains of lipids and proteins detected on erythrocytes and certain epithelial cells. The A, B and H antigens are reported to undergo modulation during malignant cellular transformation. Blood group related antigens represent a group of carbohydrate determinants carried on both glycolipids and glycoproteins. They are usually mucin type, and are detected on erythrocytes, certain epithelial cells, and in secretions of certain individuals. Sixteen genetically and biosynthetically distinct but inter related specificities belong to this group of antigens, including A, B, H, Lewis A, Lewis B, Lewis X, Lewis Y, and precursor type 1 chain antigens.Synonyms: Blood Group Antigen Lewis b
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