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Blood Group Lewis B anticorps

Reactivité: Humain IHC (p), WB, EIA Hôte: Souris Monoclonal 2-25LE unconjugated
N° du produit ABIN120686
  • Antigène Tous les produits Blood Group Lewis B
    Blood Group Lewis B
    Reactivité
    • 14
    • 11
    • 9
    Humain
    Hôte
    • 14
    Souris
    Clonalité
    • 14
    Monoclonal
    Conjugué
    • 7
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Cet anticorp Blood Group Lewis B est non-conjugé
    Application
    • 13
    • 9
    • 9
    • 5
    • 1
    • 1
    • 1
    • 1
    Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB), Enzyme Immunoassay (EIA)
    Réactivité croisée (Details)
    Species reactivity (tested):Human.
    Purification
    Affinity Chromatography on Protein A.
    Immunogène
    Mucin isolated by size exclusion chromatography from an ovarian cyst fluid (pure endocervical type according to the Fenoglio's classification)
    Clone
    2-25LE
    Isotype
    IgG1
  • Indications d'application
    Immunohistochemistry: 2-4 μg/mL for 30 min at RT. Staining of formalin-fixed tissues REQUIRES boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min. followed by cooling at RT for 20 min. Use human colon CA for postive control. Western blot. ELISA.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.
    Restrictions
    For Research Use only
  • Concentration
    1.0 mg/mL
    Buffer
    PBS, pH 7.4, with 15 mM Sodium Azide as preservative.
    Agent conservateur
    Sodium azide
    Précaution d'utilisation
    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Stock
    4 °C/-20 °C
    Stockage commentaire
    Store at 2-8 °C for 6 months. For long-term storage aliquot and store at -20 °C. Avoid freeze/thaw cycles.
    Shelf Life: one year from despatch.
    Date de péremption
    12 months
  • Antigène
    Blood Group Lewis B
    Abstract
    Blood Group Lewis B Produits
    Synonymes
    anticorps MUC5, anticorps TBM, anticorps leB, anticorps mucin 5AC, oligomeric mucus/gel-forming, anticorps MUC5AC
    Sujet
    Blood group antigens are generally defined as molecules formed by sequential addition of saccharides to the carbohydrate side chains of lipids and proteins detected on erythrocytes and certain epithelial cells. The A, B and H antigens are reported to undergo modulation during malignant cellular transformation. Blood group related antigens represent a group of carbohydrate determinants carried on both glycolipids and glycoproteins. They are usually mucin type, and are detected on erythrocytes, certain epithelial cells, and in secretions of certain individuals. Sixteen genetically and biosynthetically distinct but inter related specificities belong to this group of antigens, including A, B, H, Lewis A, Lewis B, Lewis X, Lewis Y, and precursor type 1 chain antigens.Synonyms: Blood Group Antigen Lewis b
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