ACRV1 anticorps
Aperçu rapide pour ACRV1 anticorps (ABIN120713)
Antigène
Voir toutes ACRV1 AnticorpsReactivité
Hôte
Clonalité
Conjugué
Application
Clone
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Specificité
- The antibody reacts with intra-acrosomal sperm proteins (220, 50, 34 and 21 kDa). Reacts with Human, Porcine (Pig).
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Attributs du produit
- Synonyms: Acrosomal protein SP-10, SP10, SPACA2, Acrosomal vesicle protein 1, Intra-acrosomalSperm Protein
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Purification
- Precipitation methods and size-exclusion chromatography
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Pureté
- > 95 % (by SDS-PAGE)
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Immunogène
- Freshly ejaculated human sperms were washed in PBS and extracted in 3% acetic acid,10% glycerol, 30 mM benzaminidine. The acid extract was dialyzed against 0. 2% aceticacid and subsequently used for immunization.
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Isotype
- IgM
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Indications d'application
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Immunocytochemistry: 10 μg/mL, membrane permeabilization (acetone) essential. Flow Cytometry. Western Blotting.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
Restrictions
- For Research Use only
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Concentration
- 1.0 mg/mL
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Buffer
- Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
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Agent conservateur
- Sodium azide
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Précaution d'utilisation
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Stock
- 4 °C/-20 °C
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Stockage commentaire
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Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer. Avoidrepeated freezing and thawing.
Shelf life: one year from despatch. -
Date de péremption
- 12 months
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- ACRV1 (Acrosomal Vesicle Protein 1 (ACRV1))
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Autre désignation
- ACRV1
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Sujet
- One of the most frequent causes of man infertility is defective sperm acrosome. This damage can be detected using antibodies against intra-acrosomal proteins. Besides diagnostics of sperm pathology, monoclonal antibodies against intra-acrosomal proteins can be used for evaluation of the physiological state of sperm cells as well as for selection of a suitable method of fertilization in the laboratories of assisted reproduction.Synonyms: Acrosomal protein SP-10, Acrosomal vesicle protein 1, Intra-acrosomal Sperm Protein, SP10, SPACA2
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ID gène
- 56
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UniProt
- P26436
Antigène
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