KIF5B anticorps

N° du produit ABIN120715

Cet anticorps Souris Monoclonal détecte spécifiquement KIF5B dans IF. Il présente une réactivité envers Humain, Souris, Rat et Porc.

Aperçu rapide pour KIF5B anticorps (ABIN120715)

Antigène: KIF5B (Kinesin Family Member 5B (KIF5B))

Reactivité: Humain, Souris, Rat, Porc

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp KIF5B est non-conjugé

Application: Immunofluorescence (IF)

Clone: KN-02

Détail du produit anti-KIF5B anticorps

Specificité: The antibody KN-02 recognizes heavy chain of conventional kinesin associated with vesicles and with lower affinity with denaturated molecule. Epitope is located in coiled-coil stalk domain. It stains Western blots of kinesin-enriched preparations. Epitope mapping (by limited proteolysis of partially purified porcine kinesin) followed by immunoblotting has revealed that antibodies KN-01, KN-02 and KN-03 react with different sets of fragments. The antibody KN-02 does not react with kinesin bound to taxol-stabilized microtubules.

Purification: Thiophilic adsorption-affinity chromatography

Pureté: > 95 % (by SDS-PAGE)

Immunogène: Enriched fraction of porcine brain kinesin

Isotype: IgM

Information d'application

Indications d'application: Immunocytochemistry.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL

Buffer: Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C/-20 °C

Stockage commentaire: Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf Life: one year from despatch.

Date de péremption: 12 months

Détails sur KIF5B

Antigène: KIF5B (Kinesin Family Member 5B (KIF5B))

Autre désignation: KIF5B

Sujet: Kinesin belongs to the group of microtubule-associated motor proteins known to convert chemical energy released from nucleoside triphosphates (preferentially from ATP) into mechanical energy. Conventional kinesin, member of the kinesin superfamily comprising more than 100 proteins, is involved in the anterograde vesicle transport in neuronal cells. Kinesin purified from mammalian brain homogenates is a heterotetramer consisting of two heavy (120 to 130 kDa) and two light chains (60 to 70 kDa), resulting in a molecular mass about 400 kDa. Each heavy chain contains an N-terminal globular motordomain with both a microtubule-binding site and an ATPase active center, stalk region responsible for heavy chain dimerization and finally C-terminal globular tail domain, which is implicated in cargo binding. Light chains may have a regulatory function.Synonyms: Conventional kinesin heavy chain, KIF5B, KNS, KNS1, Kinesin-1 heavy chain, UKHC, Ubiquitous kinesin heavy chain

ID gène: 3799

UniProt: P33176

Pathways: Peptide Hormone Metabolism, Ribonucleoprotein Complex Subunit Organization