OLR1 anticorps (Extracellular Domain)

N° du produit ABIN121884

Cet anticorps anti-OLR1 est un anticorps Mouton Polyclonal détectant OLR1 dans WB, IP, FACS et IHC (fro). Adapté pour Humain.

Aperçu rapide pour OLR1 anticorps (Extracellular Domain) (ABIN121884)

Antigène: OLR1 (Oxidized Low Density Lipoprotein (Lectin-Like) Receptor 1 (OLR1))

Reactivité: Humain

Hôte: Mouton

Clonalité: Polyclonal

Conjugué: Cet anticorp OLR1 est non-conjugé

Application: Western Blotting (WB), Immunoprecipitation (IP), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro))

Détail du produit anti-OLR1 anticorps

Épitope: Extracellular Domain

Purification: Affinity Chromatography

Immunogène: Recombinant human extracellular domain LOX-1 protein from bacteria

Information d'application

Indications d'application: Immunohistochemistry on frozen sections. Immunoprecipitation. Flow Cytometry: 1:10, Use 10 μL of the suggested working dilution to label 10^6 cells in 100 μL. Western Blot: 1:10 - 1/100, This antibody detects intact 50 kDa LOX-1 and also a prominent 30 kDa proteolytic fragment in western blotting.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 0.1 mg/mL

Buffer: PBS, 0.09 % Sodium Azide, 1 % BSA

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C/-20 °C

Stockage commentaire: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf Life: one year from despatch.

Date de péremption: 12 months

Détails sur OLR1

Antigène: OLR1 (Oxidized Low Density Lipoprotein (Lectin-Like) Receptor 1 (OLR1))

Autre désignation: OLR1 / LOX1

Sujet: LOX-1 is a 40-50 kDa class E scavenger receptor, expressed by endothelial cells, macrophages, activated platelets and smooth muscle cells. LOX-1 binds to a wide range of ligands, including oxidized low-density lipoprotein (oxLDL), hypochlorite modified high-density lipoprotein (HDL), aged/apoptotic cells, activated platelets and bacteria, reflecting its versatile physiological functions. Expression of the LOX-1 gene is upregulated by oxLDL and the binding of LOX-1 to oxLDL results in the activation of NF-kappaB. Furthermore, LOX-1 antibodies have been shown to suppress the oxidized HDL (oxHDL) activation of NF-KappaB in endothelial cells, suggesting that this activation may be due to the binding of oxHDL to LOX-1. Angiotensin II and the inflammatory cytokine Tumour Necrosis Factor alpha (TNFalpha) also invoke an increase in LOX-1 gene expression and studies have focused on its role in endothelial dysfunction (1,4) and inflammatory diseases such as atherosclerosis (1) and rheumatoid arthritis, as well as its affect on CD40/CD40L signaling in both atherosclerosis and human coronary artery endothelial cells (HCAECs).Synonyms: C-type lectin domain family 8 member A, CLEC8A, LOX-1, Lectin-like oxidized LDL receptor 1, Lectin-type oxidized LDL receptor 1, Ox-LDL receptor 1, Ox-LDL receptor 1 soluble form, Oxidized low-density lipoprotein receptor 1, sLOX-1, sLOX1

UniProt: P78380