Cet anticorps Lapin Polyclonal détecte spécifiquement GPR109B dans IF et IHC (p). Il présente une réactivité avec des échantillons de Humain et Souris.
Immunocytochemistry. Immunohistochemistry on formalin fixed paraffin embedded tissues: 19-38 μg/mL Antigen retrieval: Steam slides in 0.01 M sodium citrate buffer, pH 6.0, at 99-100 °C for 20 min. Remove from heat and let stand at room temperature in buffer for 20 min. Rinse in 1x TBS with Tween (TBST) for 1 min. at room temperature. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user. Further Comments: This product was originally produced by MBL International.
Restrictions
For Research Use only
Concentration
1 mg/mL
Buffer
PBS, pH 7.7, containing 0.01 % sodium azide
Agent conservateur
Sodium azide
Précaution d'utilisation
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Stock
4 °C/-80 °C
Stockage commentaire
Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -70 °C for longer. Avoid repeated freezing and thawing. Shelf Life: one year from despatch.
GPR109B/HM74 is a Nicotinic Acid GPCR. HM74 expression has been reported in adipose and spleen, as well as lymphocytes and monocytes. ESTs have been isolated from adipose, blood, liver, liver/spleen, placenta, prostate, and testis libraries.Synonyms: G-protein coupled receptor 109B, GPR109B, HM74, HM74B, NIACR2, Niacin receptor 2