CD5 anticorps (FITC)

N° du produit ABIN125750

L’anticorps Souris Monoclonal anti-CD5 a été validé pour FACS. Il convient pour détecter CD5 dans des échantillons de Humain. Il y a 3+ publications disponibles.

Aperçu rapide pour CD5 anticorps (FITC) (ABIN125750)

Antigène: CD5

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp CD5 est conjugé à/à la FITC

Application: Flow Cytometry (FACS)

Clone: CRIS1

Détail du produit anti-CD5 anticorps (FITC)

Fonction: Anti-Hu CD5 FITC

Specificité: The mouse monoclonal antibody CRIS1 reacts with an extracellular epitope of CD5, a 67 kDa single-chain transmembrane glycoprotein expressed on mature T lymphocytes, most of thymocytes and B lymphocytes subset (B-1a lymphocytes).

Réactivité croisée (Details): Human, Other species Not tested

Purification: Purified antibody is conjugated with fluorescein isothiocyanate (FITC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

Immunogène: stimulated human leukocytes

Isotype: IgG2a

Information d'application

Indications d'application: Flow cytometry: The reagent is designed for analysis of human blood cells using 20 μL reagent / 100 μL of whole blood or 10⁶ cells in a suspension. The content of a vial (2 ml) is sufficient for 100 tests.

Restrictions: For Research Use only

Stockage

Reconstitution: No reconstitution is necessary.

Buffer: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Do not freeze.
Avoid prolonged exposure to light.

Stock: 4 °C

Stockage commentaire: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

Références pour anticorps anti-CD5 (FITC) (ABIN125750)

Guarné, Bravo, Calvo, Lozano, Vives, Fita: "Conformation of the hypervariable region L3 without the key proline residue." dans: Protein science : a publication of the Protein Society, Vol. 5, Issue 1, pp. 167-9, (1996) (PubMed).

Alberola-Ila, Places, Cantrell, Vives, Lozano: "Intracellular events involved in CD5-induced human T cell activation and proliferation." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 148, Issue 5, pp. 1287-93, (1992) (PubMed).

Arrizabalaga, Mirapeix, Darnell, Torras, Revert: "Cellular immunity analysis using monoclonal antibodies in human glomerulonephritis." dans: Nephron, Vol. 53, Issue 1, pp. 41-9, (1989) (PubMed).

Détails sur CD5

Antigène: CD5

Autre désignation: CD5

Sujet: CD5 Molecule,CD5 antigen (T1, 67 kDa) is a human cell surface T-lymphocyte single-chain transmembrane glycoprotein. CD5 is expressed on all mature T-lymphocytes, most of thymocytes, subset of B-lymphocytes and on many T-cell leukemias and lymphomas. It is a type I membrane glycoprotein whose extracellular region contains three scavenger receptor cysteine-rich (SRCR) domains. The CD5 is a signal transducing molecule whose cytoplasmic tail is devoid of any intrinsic catalytic activity. CD5 modulates signaling through the antigen-specific receptor complex (TCR and BCR). CD5 crosslinking induces extracellular Ca++ mobilization, tyrosine phosphorylation of intracellular proteins and DAG production. Preliminary evidence shows protein associations with ZAP-70, p56lck, p59fyn, PC-PLC, etc. CD5 may serve as a dual receptor, giving either stimulatory or inhibitory signals depending both on the cell type and development stage. In thymocytes and B1a cells it seems to provide inhibitory signals, in peripheral mature T lymhocytes it acts as a costimulatory signal receptor. CD5 is the phenotypic marker of a B cell subpopulation involved in the production of autoreactive antibodies. Disease relevance: CD5 is a phenotypic marker for some B cell lymphoproliferative disorders (B-CLL, Hairy cell leukemia, etc.). The CD5+ popuation is expanded in some autoimmune disorders (rheumatoid arthritis, etc.). Herpes virus infections induce loss of CD5 expression in the expanded CD8+ human T cells.,T1, LEU1

ID gène: 921

UniProt: P06127