Orf1 (N. Meningitidis) anticorps

N° du produit ABIN125757

Cet anticorps anti- est un anticorps Lapin Polyclonal détectant dans WB. Adapté pour Neisseria meningitidis. Ce Primary Antibody a été cité dans 1 publication.

Aperçu rapide pour Orf1 (N. Meningitidis) anticorps (ABIN125757)

Antigène: Orf1 (N. Meningitidis)

Reactivité: Neisseria meningitidis

Hôte: Lapin

Clonalité: Polyclonal

Application: Western Blotting (WB)

Détail du produit

Fonction: Anti-Orf1 (N. meningitidis) Purified

Specificité: The polyclonal antibody reacts with Orf1 (FrpD), a 28 - 32 kDa Neisseria meningitidis outer membrane lipoprotein. Orf1 is highly conserved in a set of meningococcal strains representative of all serogroups and does not exhibit any similarity to known sequences of other organisms.

Réactivité croisée (Details): Neisseria meningitidis

Purification: Purified by ligand affinity chromatography.

Pureté: > 95 % (by SDS-PAGE)

Immunogène: Slices containing purified FrpD250-6xHis-tagged protein were excised from 10% SDS-PAGE gels, grinded, mixed with 1 mg/ml of aluminum hydroxide in PBS, and used to immunize a rabbit.

Information d'application

Indications d'application: Western blotting: Recommended dilution 0.5-1 μg/mL, reducing conditions.

Restrictions: For Research Use only

Stockage

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Do not freeze.

Stock: 4 °C

Stockage commentaire: Store at 2-8°C. Do not freeze.

Référence

Prochazkova, Osicka, Linhartova, Halada, Sulc, Sebo: "The Neisseria meningitidis outer membrane lipoprotein FrpD binds the RTX protein FrpC." dans: The Journal of biological chemistry, Vol. 280, Issue 5, pp. 3251-8, (2005) (PubMed).

Détail du antigène

Antigène: Orf1 (N. Meningitidis)

Sujet: Orf1 (FrpD) is a Neisseria meningitidis outer membrane lipoprotein. Subcellular localization and [3H]palmitic acid labeling in Escherichia coli revealed that FrpD is synthesized with a type II signal peptide for export across the cytoplasmic membrane and is, upon processing to a lipoprotein, sorted to the outer bacterial membrane.,FrpD