Vitellogenin 2 anticorps

N° du produit ABIN125873

L’anticorps anti-Vitellogenin 2 Polyclonal Lapin est utilisé pour la détection de Vitellogenin 2 dans des échantillons de Sea bass (Dicentrarchus labrax), Senegalese sole et Sparus aurata. Il a été validé pour ELISA et WB.

Aperçu rapide pour Vitellogenin 2 anticorps (ABIN125873)

Antigène: Vitellogenin 2

Reactivité: Sea bass (Dicentrarchus labrax), Senegalese sole, Sparus aurata 

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Inconjugué

Application: ELISA, Western Blotting (WB)

Détail du produit

Fonction: Anti-VTG | Sole vitellogenin

Réactivité croisée (Details): Senegalese sole, Dicentrarchus labrax (sea bass), Sparus aurata (seabream)
Not reactive in: No confirmed exceptions from predicted reactivity are currently known

Purification: Serum

Immunogène: Native vitellogenin purified from plasma of estradiol induced male of Senegalese sole (Solea senegalensis)

Information d'application

Indications d'application: 1 : 5 000 on sole serum (ELISA), 1 : 5 000 (WB)

Commentaires:

the developed VTG ELISA, using these VTG and AbVTG, has been validated for Senegalese sole, sea bass (Dicentrarchus labrax) and seabream (Sparus aurata), which all gave parallel displacement curves in the assay. Probably, plasmas from several other fish species displace parallel and can also be used in the assay, although it has to be validated for each case.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: For reconstitution add 200 μL of sterile water

Buffer: Serum

Conseil sur la manipulation: Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube

Stock: -20 °C

Stockage commentaire: Store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Détail du antigène

Antigène: Vitellogenin 2

Autre désignation: VTG

Sujet:

Background: Vitellogen (VTG) is a glucolipoprotein yolk precursor produced by all oviparous animals. This protein is female-specific and its expression is under hormonal control (estrogen). However, in the presence of Endocrine Disrupting Chemicals (EDC's), males can express the VgTG gene in a dose dependent manner. The use of VTG gene expression in male fish can be used as a molecular marker of exposure to estrogenic EDC's.

Additional information: VTG can be purified using following methodology: Mañanós et al. (1994). Sea bass (Dicentrarchus labrax L.) vitellogenin. I-Induction, purification and partial characterization. Comparative Biochemistry and Physiology Part B: Comparative Biochemistry, Vol 107 (2): 205-216.Guzman et al. (2008) Vitellogenin, steroid plasma levels and spawning performance of cultured female Senegalese sole (Solea senegalensis).Gen and Comp Endocrinology 156: 285-297.

Poids moléculaire: ca. 200 kDa