M13 Bacteriophage G8P Capsid anticorps (FITC)

N° du produit ABIN125966

L’anticorps Souris Monoclonal anti- a été validé pour PhD. Il convient pour détecter dans des échantillons de Bacteriophage M13. Il y a 1 publication disponible.

Aperçu rapide pour M13 Bacteriophage G8P Capsid anticorps (FITC) (ABIN125966)

Antigène: M13 Bacteriophage G8P Capsid

Reactivité: Bacteriophage M13

Hôte: Souris

Clonalité: Monoclonal

Conjugué: FITC

Application: Phage Display (PhD)

Clone: B62-FE2

Détail du produit

Specificité: B62-FE2 binds to an epitope on pVIII (phage coat protein) covering the N-terminal region of g8p AEGDDPAKAAFDSLQASAT (See Kneissel et al.).

Purification: Affinity Chromatography on Protein A

Immunogène: fd phages from E. coli F+ strain (JM109).

Isotype: IgG2b

Information d'application

Indications d'application: Phage Display (Immunoassays for the identification of recombinant antigen- orantibody-phages): use at 1 μg/mL.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.4 mg/mL

Buffer: Tris buffer, pH 8.0, 0.09 % Sodium Azide, 0.5 % BSA

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Store the antibody undiluted at 2-8 °C, reconstitute and store at 2-8 °C for one month or at -20 °C for longer. For extended storage reconstitute product with 50% Glycerol instead of water and then aliquot contents and freeze at -20 °C or below. Centrifuge product if not completely clear after standing at room temperature.

Référence

Phumyen, Jumnainsong, Leelayuwat: "Improved binding activity of antibodies against major histocompatibility complex class I chain-related gene A by phage display technology for cancer-targeted therapy." dans: Journal of biomedicine & biotechnology, Vol. 2012, pp. 597647, (2013) (PubMed).

Détail du antigène

Antigène: M13 Bacteriophage G8P Capsid

Classe de substances: Phage Protein

Sujet: M13 is a filamentous bacteriophage composed of circular single stranded DNA (ssDNA) which is 6407 nucleotides long encapsulated in approximately 2700 copies of the major coat protein P8, and capped with 5 copies of two different minor coat proteins (P9, P6, P3) on the ends. The minor coat protein P3 attaches to the receptor at the tip of the F pilus of the host Escherichia coli. Infection with filamentous phages is not lethal, however the infection causes turbid plaques in E. coli. It is a non-lytic virus. However a decrease in the rate of cell growth is seen in the infected cells. Antibodies to M13 filamentous phage coat proteins are instrumental in the selection and detection of phages expressing specific antibody fragments or peptide sequences at their surface. The display of repertoires of antibody fragments on the surface of filamentous phage offers a new way to produce immunoreagents with defined specificities. Phage derived antibody fragments offer a number of advantages over mouse monoclonal antibodies, such as better clearance from the blood, the possibility to select from human combinatorial libraries and the relative ease by which such fragments can be manipulated. The phage display technique thus facilitates the selection of antibody fragments of therapeutic value or research interest. Antibodies to M13 filamentous phage coat proteins are instrumental in the selection and detection of phages expressing specific antibody fragments or peptide sequences at their surface.Synonyms: Coat protein B, Gene 8 protein, Major coat protein

ID gène: 927333

NCBI Accession: NP_510890

UniProt: P69541