Collagen Type I anticorps

N° du produit ABIN126040

Cet anticorps Lapin Polyclonal détecte spécifiquement Collagen Type I dans IHC (fro), IF et EIA. Il présente une réactivité envers Humain.

Aperçu rapide pour Collagen Type I anticorps (ABIN126040)

Antigène: Collagen Type I (COL1) (Collagen, Type I (COL1))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp Collagen Type I est non-conjugé

Application: Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Enzyme Immunoassay (EIA)

Détail du produit anti-Collagen Type I anticorps

Specificité: This antibody detects exhibits the following reactivities in ELISA both native and denaturated Collagen types: Collagen types 1 and 3: 100 % . Collagen types 4 and 5: 90 %. Collagen type 2: 80 %. Fibronectin, Albumin and Immunoglobulins all < 0.5 %.

Réactivité croisée (Details): Also reacts to: type II, and V, III, IV

Purification: Affinity Chromatography on immobilized collagen types I-V

Immunogène: A mixture of human collagen 1-5 from human placenta.

Information d'application

Indications d'application: Immunohistochemistry on Frozen Sections (1/10-1/40). ELISA (1/2000-1/8000). Immunoflourescence (1/10-1/40).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL

Buffer: PBS, pH 7.5 containing 0.1 % Mannitol and 0.1 % Dextran and 0.09 % Sodium Azide as preservative.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Détails sur Collagen Type I

Antigène: Collagen Type I (COL1) (Collagen, Type I (COL1))

Autre désignation: Collagen Type I

Sujet: Collagen I is a member of group I collagen (fibrillar forming collagen), it forms the fibrils of tendon, ligaments and bones. In bones the fibrils are mineralized with calcium hydroxyapatite. Collagen II is specific for cartilaginous tissues. It is essential for the normal embryonic development of the skeleton, for linear growth and for the ability of cartilage to resist compressive forces. High expression of isoform 2 in juvenile chondrocyte and low in fetal chondrocyte. Collagen III occurs in most soft connective tissues along with collagen I. Collagen IV is the major structural component of glomerular basement membranes, forming a Chicken-wire meshwork together with laminins, proteoglycans and entactin/nidogen. Collagen V is a member of group I collagen (fibrillar forming collagen). It is a minor connective tissue component of nearly ubiquitous distribution. Collagen V binds to DNA, heparan sulfate, thrombospondin, heparin, and insulin.Synonyms: Alpha-1 type I collagen, Alpha-2 type I collagen, COL1A1, COL1A2

ID gène: 1277

NCBI Accession: NP_000079

UniProt: P02452