Phosphoserine anticorps (phosphorylated)

N° du produit ABIN126864

Cet anticorps anti-Phosphoserine Monoclonal Souris (Clone 7F12) (ABIN126864) détecte spécifiquement Phosphoserine dans WB, IP et EIA.

Aperçu rapide pour Phosphoserine anticorps (phosphorylated) (ABIN126864)

Antigène: Phosphoserine

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Phosphoserine est non-conjugé

Application: Western Blotting (WB), Immunoprecipitation (IP), Enzyme Immunoassay (EIA)

Clone: 7F12

Détail du produit anti-Phosphoserine anticorps

Épitope: phosphorylated

Réactivité croisée (Details): Species reactivity (tested):Human, Mouse, Rat and Dog.

Purification: Subsequent Thiophilic Adsorption and Size Exclusion Chromatography

Ingrédients: incl. positive Control

Immunogène: Synthetic phosphopeptide conjugated to KLH

Isotype: IgG1

Information d'application

Indications d'application: Western Blot: 1 μg/mL for HRPO/ECL detection. Recommended blocking buffer: BSA/Tween 20 based blocking buffer. DO NOT USE MILKOR CASEIN FOR BLOCKING! ELISA: 0.05 μg/mL. Immunoprecipitation: 1-10 μg per 10^6 pervanadate-treated A431 cells.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Protocole: Positive control: pSer / pThr Molecular Weight MarkerFormulation:

Restrictions: For Research Use only

Stockage

Reconstitution: Restore with 1.0 mL H2O (15 min, RT)

Buffer: 1 mL2 x PBS containing 0.09 % Sodium Azide, PEG and Sucrose

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C/-80 °C

Stockage commentaire: Store lyophilized (preferably in a desiccator) at -20 °C and reconstituted (aliquote andfreeze in liquid nitrogen) at -80 °C. Thaw aliquots at 37 °C. Thawed aliquots may be stored at 4 °C up to 3 months. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.

Date de péremption: 12 months

Détails sur Phosphoserine

Antigène: Phosphoserine

Classe de substances: Amino Acid

Sujet: Phosphorylation and dephosphorylation of cellular proteins are central steps in transducing extracellular signals to the cell nucleus. Phosphorylated epitopes may serve as docking sites for the assembly of protein complexes or may alter the 3-dimensional protein structure thus modulating enzymatic activity or the ability to undergo protein-protein-interactions. Modification of proteins on serine residues is mediated by serine/threonine kinases.