Phosphothreonine anticorps (phosphorylated)

N° du produit ABIN126868

Cet anticorps Souris Monoclonal détecte spécifiquement Phosphothreonine dans WB, IP et EIA.

Aperçu rapide pour Phosphothreonine anticorps (phosphorylated) (ABIN126868)

Antigène: Phosphothreonine

Reactivité: Veuillez nous consulter SVP

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Phosphothreonine est non-conjugé

Application: Western Blotting (WB), Immunoprecipitation (IP), Enzyme Immunoassay (EIA)

Clone: 4D11

Détail du produit anti-Phosphothreonine anticorps

Épitope: phosphorylated

Purification: Size Exclusion Chromatography.

Ingrédients: incl. positive Control

Immunogène: Synthetic phosphopeptide conjugated to KLH.

Isotype: IgM

Information d'application

Indications d'application: Immunoblotting (Western Blot): 1 μg/mL for HRPO/ECL detection. Recommended blocking buffer: BSA/Tween 20 basedblocking buffer. DO NOT USE MILK OR CASEIN FOR BLOCKING! ELISA: 0.05 μg/mL. Immunoprecipitation: 1-10 μg per 10^6 pervanadate-treated A431 cells. Positive Control: Phosphoserine/Phosphothreonine.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Protocole: Positive Control: pSer / pThr Molecular Weight MarkerFormulation: The pSer/pThr molecular weight marker contains rabbit musclephosphoproteins isolated by Fe3+/IDA - affinity chromatography. Proteins are lyophilizedfrom PBS/NaF/PEG/Sucrose/ Bromophenolblue and Na - azide. After reconstitution thesolution contains 0. 09% Na-azide. Stability: Reconstitute by addition of 200 µl H2O. After complete solubilization add 200 µl2x SDS-PAGE sample buffer, mix and incubate at 90°C for 5 min. Application: The pSer/pThr molecular weight marker is recommended for immunoblotapplications. Use 20µl molecular weight marker per lane. Note: Use BSA based blotincubation buffers. Milk, Casein and Blotto might interfere with antibody - antigeninteraction. Storage: Aliquote and store frozen. Avoid repeated freeze/thaw cycles. Shelf life: one year from despatch.

Restrictions: For Research Use only

Stockage

Reconstitution: Restore with 1 mL H2O (15 min, RT).

Buffer: 1 mL2 x PBS / 0.09 % Sodium Azide / PEG and Sucrose.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C/-80 °C

Stockage commentaire: Store lyophilized (preferably in a desiccator) at -20 °C and reconstituted (aliquote andfreeze in liquid nitrogen) at -80 °C. Avoid repeated freezing and thawing. Thaw aliquots at 37 °C. Thawed aliquots may be stored at 4 °C up to 1 week.
Shelf life: one year from despatch.

Date de péremption: 12 months

Détails sur Phosphothreonine

Antigène: Phosphothreonine

Classe de substances: Amino Acid

Sujet: Phosphorylation and dephosphorylation of cellular proteins are central steps in transducing extracellular signals to the cell nucleus. Phosphorylated epitopes may serve as docking sites for the assembly of protein complexes or may alter the 3-dimensional protein structure thus modulating enzymatic activity or the ability to undergo protein-protein-interactions. Modification of proteins on serine residues is mediated by serine/threonine kinases.