Vimentin anticorps
Aperçu rapide pour Vimentin anticorps (ABIN126914)
Antigène
Voir toutes Vimentin (VIM) AnticorpsReactivité
Hôte
Clonalité
Conjugué
Application
Clone
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Specificité
- This antibody specifically recognizes Vimentin at 54 kDa in Western-blot applications.
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Purification
- Size exclusion chromatography
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Ingrédients
- incl. positive Control
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Immunogène
- Synthetic peptide conjugated to hemocyanin
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Isotype
- IgG
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Indications d'application
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Western Blot: 0.5 μg/mL for HRPO/ECL detection. Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubationbuffer.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
Restrictions
- For Research Use only
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Reconstitution
- Restore with 1 mL H2O (15 min, RT).
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Buffer
- 1 mL2 x PBS containing 0.09 % Sodium Azide/PEG and Sucrose.
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Agent conservateur
- Sodium azide
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Précaution d'utilisation
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Stock
- -20 °C/-80 °C
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Stockage commentaire
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Store lyophilized (preferably in a desiccator) at -20 °C and reconstituted (aliquote andfreeze in liquid nitrogen) at -80 °C. Avoid repeated freezing and thawing. Thaw aliquots at 37 °C. Thawed aliquots may be stored at 4 °C up to 3 months.
Shelf life: one year from despatch. -
Date de péremption
- 12 months
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- Vimentin (VIM)
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Autre désignation
- Vimentin
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Sujet
- Vimentin is a major subunit protein of intermediate filaments found in mesenchymal cells. Vimentin is expressed in many tumors/tumor cell lines after epithelial to mesenchymal transition.Synonyms: VIM
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Poids moléculaire
- 54 kDa
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ID gène
- 7431
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UniProt
- P08670
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Pathways
- Caspase Cascade in Apoptosis
Antigène
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