HRK anticorps

N° du produit ABIN127035

Détail du produit anti-HRK anticorps

Antigène: HRK (Harakiri, BCL2 Interacting Protein (Contains Only BH3 Domain) (HRK))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp HRK est non-conjugé

Application: Western Blotting (WB)

Specificité: The antibody detects Hrk fused with green fluorescent protein in crude extracts of E. coli transfected with Hrk-GFP.

Purification: Affinity purified

Immunogène: Synthetic peptide corresponding to residues surrounding amino acids 50 of human HRK.

Information d'application

Indications d'application: Western Blot: 1 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 0.5 mg/mL

Buffer: PBS, pH 7.2, containing 50 % glycerol, 1 % BSA, 0.02 % sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: -20 °C/-80 °C

Stockage commentaire: Store the antibody undiluted at -20 °C or for long term storage (in aliquots) at -70 °C.

Détails sur HRK

Antigène: HRK (Harakiri, BCL2 Interacting Protein (Contains Only BH3 Domain) (HRK))

Autre désignation: Harakiri / HRK (HRK Produits)

Synonymes: anticorps DP5, anticorps HARAKIRI, anticorps AI838259, anticorps Bid3, anticorps harakiri, anticorps Dp5, anticorps harakiri, BCL2 interacting protein, anticorps harakiri, BCL2 interacting protein (contains only BH3 domain), anticorps HRK, anticorps Hrk

Sujet: Apoptosis plays a major role in normal organism development, tissue homeostasis, and removal of damaged cells. Hrk, a pro-apoptotic member of the Bcl-2 homology domain-3 (BH3) group of the Bcl-2 family of proteins, was identified as a novel protein induced during programmed neuronal death. It lacks significant homology to other Bcl-2 family members except for an 8-amino acid region that is similar to the BH3 motif of Bik. Hrk regulates apoptosis through interaction with the anti-apoptotic proteins Bcl-2 and Bcl-XL via this domain. It does not interact with the pro-apoptotic proteins Bax, Bak, or Bcl-XS. Hrk localizes to mitochondrial membranes in a pattern similar to that previously reported for Bcl-2 and Bcl-XL. Despite its predicted molecular weight, Hrk often migrates at 12-15 kDa.Synonyms: Activator of apoptosis harakiri, BH3-interacting domain-containing protein 3, BID3, Neuronal death protein DP5

ID gène: 8739

NCBI Accession: NP_003797

UniProt: O00198