Cathepsin B anticorps

N° du produit ABIN127046

L’anticorps anti-Cathepsin B Polyclonal Lapin est utilisé pour la détection de Cathepsin B dans des échantillons de Humain, Souris et Rat. Il a été validé pour WB et IHC (p).

Aperçu rapide pour Cathepsin B anticorps (ABIN127046)

Antigène: Cathepsin B (CTSB)

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp Cathepsin B est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Détail du produit anti-Cathepsin B anticorps

Specificité: The purified antibody detects both the proform (37-43 kDa) and the mature form (25 kDa) of Cathepsin B.

Réactivité croisée (Details): Species reactivity (tested):Human, Mouse and Rat.

Purification: Affinity Chromatography.

Immunogène: Synthetic peptide surrounding amino acid 322 of human cathepsins B

Information d'application

Indications d'application: Western Blot: 0.5-4 μg/mL. Immunohistochemistry: 10-20 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 0.2 mg/mL

Buffer: PBS, pH 7.2 containing 30 % Glycerol, 0.5 % BSA and 0.01 % Thimerosal.

Agent conservateur: Thimerosal (Merthiolate)

Précaution d'utilisation: This product contains thimerosal (merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: -20 °C/-80 °C

Stockage commentaire: Store the antibody undiluted at -20 °C or for long term storage (in aliquots) at -20 °C to -70 °C.

Détails sur Cathepsin B

Antigène: Cathepsin B (CTSB)

Autre désignation: Cathepsin B

Sujet: Cathepsin B is a normal lysosomal cysteine proteinase that is expressed in all cells. It is synthesized as an inactive 43 kDa pro-enzyme which, by removal of a 62 amino acid propeptide, is activated to the single-chain form of 31 kDa or the two-chain form (25 and 5 kDa subunits). Matured cathepsin B is normally localized in the lysosomes where it functions in protein turnover. Cathepsin B is also shown capable of degrading extracellular matrix proteins. Elevated levels of cathepsin B correlate with malignancy suggesting that this enzyme may be a useful prognostic marker for several types of human cancers.Synonyms: APP secretase, APPS, CPSB, CTSB, Cathepsin B1

ID gène: 1508

NCBI Accession: NP_001899

UniProt: P07858

Pathways: Activation of Innate immune Response, Toll-Like Receptors Cascades