MAPK8 / MAPK9 (pThr183), (pTyr185) anticorps

N° du produit ABIN127216

Cet anticorps Lapin Polyclonal détecte spécifiquement MAPK8 / MAPK9 dans WB, IHC (p), IF et IP. Il présente une réactivité avec des échantillons de Humain, Souris et Rat.

Aperçu rapide pour MAPK8 / MAPK9 (pThr183), (pTyr185) anticorps (ABIN127216)

Antigène: MAPK8 / MAPK9

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Inconjugué

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF), Immunoprecipitation (IP)

Détail du produit

Épitope: pThr183, pTyr185

Purification: Affinity Chromatography.

Immunogène: Synthetic phospho-peptide corresponding to residues surrounding Thr183/Tyr185 of Human SAPK/JNK

Information d'application

Indications d'application: Western Blot: 1-2 μg/mL. Immunoprecipitation: 10-20 μg/mL. Immunocytochemistry: 10-20 μg/mL. Immunohistochemistry: 20-30 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 0.5 mg/mL

Buffer: PBS, pH 7.2, containing 50 % Glycerol, 1 % BSA and 0.02 % Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: -20 °C/-80 °C

Stockage commentaire: Store the antibody undiluted at -20 °C or for long term storage (in aliquots) at -70 °C.

Détail du antigène

Antigène: MAPK8 / MAPK9

Sujet: The JNK pathway is activated by largely distinct stimuli including inflammatory cytokines (such as TNF-alpha and IL-1), UV light, inhibitors of protein synthesis and osmotic stress. Activated MEKK1 phosphorylates SEK1 (also known as MKK4), which in turn activates SAPK (also known as JNK). JNK binds tightly to the N-terminal region of c-Jun and ATF-2, and phosphorylates c-Jun at Ser63 and Ser73 and ATF-2 at Thr69 and Thr71.Synonyms: JNK-1, JNK-2, JNK-46, JNK-55, JNK1, JNK2, MAPK-8, MAPK-9, MAPK8, MAPK9, Mitogen-activated protein kinase 8/9, PRKM8, PRKM9, SAPK, Stress-activated protein kinase JNK11/JNK2, c-Jun N-terminal kinase 1/2