Selectin E/CD62e anticorps

N° du produit ABIN127258

Cet anticorps Lapin Polyclonal détecte spécifiquement Selectin E/CD62e dans WB, IP et IHC (p). Il présente une réactivité envers Humain, Souris et Rat.

Aperçu rapide pour Selectin E/CD62e anticorps (ABIN127258)

Antigène: Selectin E/CD62e (SELE) (Selectin E (SELE))

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp Selectin E/CD62e est non-conjugé

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Détail du produit anti-Selectin E/CD62e anticorps

Purification: Protein A Affinity Chromatography

Immunogène: Synthetic peptide surrounding amino acid 584 of Human E-Selectin.

Isotype: IgG

Information d'application

Indications d'application: Western Blot: 0.5-4 μg/mL. Immunoprecipitation: 10-20 μg/mL. Immunohistochemistry (Paraffin Sections): 10-20 μg/mL. Positive Controls: Jurkat cell lysate, Mouse small intestine and Rat kidney tissue lysate.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 0.5 mg/mL

Buffer: PBS containing 0.02 % Thimerosal as preservative

Agent conservateur: Thimerosal (Merthiolate)

Précaution d'utilisation: This product contains thimerosal (merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: -20 °C/-80 °C

Stockage commentaire: Store the antibody undiluted at -20 °C or for long term storage (in aliquots) at -20 °C to -70 °C.

Détails sur Selectin E/CD62e

Antigène: Selectin E/CD62e (SELE) (Selectin E (SELE))

Autre désignation: CD62E / E-Selectin

Sujet: Selectin E is also known as endothelial leukocyte adhesion molecule 1 (ELAM1) or CD62E. It is a type I membrane protein that belongs to the selectin family of surface molecules (along with CD62L and CD62P). Selectins are C type cell surface lectins that play a role in leukocyte adhesion to the blood vessel wall endothelium. CD62E (E selectin) is an endothelial cell specific selectin that is expressed on cytokine induced endothelial cells only after activation with proinflammatory cytokines. In vitro experiments have shown that IL1, TNFalpha and bacterial wall components like lipopolysaccharides induce the transcription of CD62E in an NFkB dependent signalling cascade. CD62E has been associated with blood vessel endothelium in diverse inflammatory situations. The main ligands recognised by CD62E are oligosaccharides related to sialyl lewis x.Synonyms: CD62 antigen-like family member E, ELAM1, Endothelial leukocyte adhesion molecule 1, LECAM2, Leukocyte-endothelial cell adhesion molecule 2, SELE

ID gène: 9606

UniProt: P16581

Pathways: Thromboxane A2 Receptor Signaling