BUB1 anticorps (AA 281-419)
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- Antigène Voir toutes BUB1 Anticorps
- BUB1 (Budding Uninhibited By Benzimidazoles 1 Homolog (Yeast) (BUB1))
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Épitope
- AA 281-419
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Reactivité
- Humain
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Hôte
- Souris
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Clonalité
- Monoclonal
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Conjugué
- Cet anticorp BUB1 est non-conjugé
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Application
- Western Blotting (WB), Immunoprecipitation (IP)
- Attributs du produit
- Concentration Definition: by UV absorbance at 280 nm
- Immunogène
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This protein A purified monoclonal antibody was produced by repeated immunizations with a recombinant protein corresponding to amino acid residues 281-419 of human BUB1 protein.
Immunogentype:Recombinant - Clone
- 14H5
- Isotype
- IgG
- Top Product
- Discover our top product BUB1 Anticorps primaire
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- Indications d'application
- This protein A purified antibody has been tested for use in immunoprecipitation, immunofluorescence staining and western blot and is capable of detecting endogenous protein. Specific conditions for reactivity should be optimized by the end user. Expect a predominant band at ~ 160 kDa corresponding to full-length protein by western blotting in the appropriate cell lysate or extract. The use of HeLa whole cell lysates prepared using a RIPA buffer is recommended as a positive control. For IF microscopy use cells grown on cover slips fixed with 3.5% paraformaldehyde in PBS at pH 6.8 OR 100% methanol at -20° C. Permeabilize fixed cells with 0.5% Triton X-100.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 1.0 mg/mL
- Buffer
- 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
- Agent conservateur
- Sodium azide
- Précaution d'utilisation
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Stock
- -20 °C
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- Antigène
- BUB1 (Budding Uninhibited By Benzimidazoles 1 Homolog (Yeast) (BUB1))
- Autre désignation
- BUB1 (BUB1 Produits)
- Synonymes
- anticorps bub1a, anticorps bub1l, anticorps hbub1, anticorps xbub1, anticorps BUB1A, anticorps BUB1L, anticorps hBUB1, anticorps AL022991, anticorps Bub1a, anticorps C80208, anticorps D2Xrf87, anticorps BUB1 mitotic checkpoint serine/threonine kinase, anticorps BUB1, mitotic checkpoint serine/threonine kinase, anticorps BUB1, anticorps bub1, anticorps Bub1
- Sujet
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BUB1 (also called Mitotic checkpoint serine/threonine-protein kinase BUB1and BUB1A) is a kinase involved in spindle checkpoint function. The kinase functions in part by phosphorylating BUB3, a member of the miotic checkpoint complex, and activating the spindle checkpoint. Mutations in this gene have been associated with aneuploidy and several forms of cancer. BUB1 is autophosphorylated when cells enter mitosis. This protein is localized to the nucleus in interphase cells. Kinetochore localization is required for normal mitotic timing and checkpoint response to spindle damage. BUB1 is highly expressed in testis and thymus, less in colon, spleen, lung and small intestine. Expression is associated with cells/tissues with a high mitotic index.
Synonyms: Budding uninhibited by benzimidazoles 1 homolog antibody, hBUB1 antibody, Mitotic checkpoint serine/threonine protein kinase BUB1 antibody - ID gène
- 699
- NCBI Accession
- NP_004327
- UniProt
- Q53QE4
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