NEK2 anticorps (AA 275-300)

N° du produit ABIN129582

N° du produit (Fournisseur): 600-401-452

Cet anticorps anti-NEK2 est un anticorps Lapin Polyclonal détectant NEK2 dans WB, ELISA et FM. Adapté pour Souris.

Aperçu rapide pour NEK2 anticorps (AA 275-300) (ABIN129582)

Antigène: NEK2 (NIMA (Never in Mitosis Gene A)-Related Kinase 2 (NEK2))

Reactivité: Souris

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp NEK2 est non-conjugé

Application: Western Blotting (WB), ELISA, Fluorescence Microscopy (FM)

Détail du produit anti-NEK2 anticorps

Épitope: AA 275-300

N° du produit (Fournisseur): 600-401-452

Fournisseur: Rockland

Fonction: NEK2 Antibody

Réactivité croisée (Details): BLAST analysis indicates 100 % homology of the immunizing sequence with NEK2 homologues from human and chimpanzee.

Attributs du produit: Synonyms: rabbit anti-NEK2 antibody, Serine threonine protein kinase Nek2 antibody, HSPK 21 antibody, NEK 2 antibody, NEK2A antibody, NIMA, never in mitosis a-related kinase 2 antibody, NimA-like protein kinase 1 antibody, NIMA related kinase 2 antibody, NEK-2, NLK1, NLK-1, NEK2A, HSPK21

Purification: This product is an affinity-purified antibody produced by immunoaffinity chromatography using peptide coupled to agarose beads.

Stérilité: Sterile filtered

Immunogène:

Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region near aa 275-300 of Human NEK2 protein.

Immunogen Type: Conjugated Peptide

Isotype: IgG

Information d'application

Indications d'application:

Application Note: This affinity purified antibody has been tested for use in ELISA and by western blot.  Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 52 kDa in size corresponding to NEK2 protein by western blotting in the appropriate cell lysate or extract.  Splice variants exist for this protein that may result in the detection of lower molecular weight bands.

Western Blot Dilution: 1:500 - 1:2,000

ELISA Dilution: 1:2,000 - 1:10,000

IF Microscopy Dilution: User Optimized

Other: User Optimized

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Date de péremption: 12 months

Détails sur NEK2

Antigène: NEK2 (NIMA (Never in Mitosis Gene A)-Related Kinase 2 (NEK2))

Autre désignation: NEK2

Sujet: Background: NEK2, also called serine/threonine-protein kinase Nek2NimA-related protein kinase 2, NimA-like protein kinase 1 and HSPK 21, is a protein kinase involved in mitotic regulation that may have a role at the G₂-M transition and may also play a role in meiosis. NEK2 has a nuclear localization and accumulates throughout S phase and shows maximal levels in late G₂. This expression pattern is highly reminiscent of that of A and B cyclins.

ID gène: 4751, 57791147

UniProt: P51955

Pathways: M Phase