DNMT3L anticorps (AA 150-175)

N° du produit ABIN129584

N° du produit (Fournisseur): 600-401-454

L’anticorps Lapin Polyclonal anti-DNMT3L a été validé pour WB, ELISA, IHC et IP. Il convient pour détecter DNMT3L dans des échantillons de Humain.

Aperçu rapide pour DNMT3L anticorps (AA 150-175) (ABIN129584)

Antigène: DNMT3L (TRDMT1) (DNA (Cytosine-5-)-Methyltransferase 3 Like (TRDMT1))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp DNMT3L est non-conjugé

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunoprecipitation (IP)

Détail du produit anti-DNMT3L anticorps

Épitope: AA 150-175

N° du produit (Fournisseur): 600-401-454

Fournisseur: Rockland

Fonction: DNMT3L Antibody

Réactivité croisée (Details): This antibody is expected to cross-react with mouse as the sequence of the human immunogen shows 76 % identity with mouse.

Attributs du produit: Synonyms: rabbit anti-DNMT3L antibody, DNA (cytosine-5)-methyltransferase 3-like, DNMT3L, DNMT 3L, DNMT-3L

Purification: This is an affinity purified antibody produced by immunoaffinity chromatography using the immunizing peptide after immobilization to a solid phase.

Stérilité: Sterile filtered

Immunogène:

Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region near aa 150-175 of Human DNMT3L (DNA (cytosine-5-)-methyltransferase 3-like). DNMT3L is a nuclear protein with similarity to DNA methyltransferases.

Immunogen Type: Conjugated Peptide

Isotype: IgG

Information d'application

Indications d'application:

Immunohistochemistry Dilution: 1:500

Application Note: Anti-DNMT3L antibody has been tested for use in ELISA against the immunizing peptide.  This antibody has been tested in immunohistochemistry and western blot. This antibody works for western blotting only following immunoprecipitation.  The expected molecular weight of human DNMT3L is 43.6 kDa. Reactivity in other immunoassays is unknown.

Western Blot Dilution: 1:500

Immunoprecipitation Dilution: 1:100

ELISA Dilution: 1:1000 - 1:3000

Other: User Optimized

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1.11 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Date de péremption: 12 months

Détails sur DNMT3L

Antigène: DNMT3L (TRDMT1) (DNA (Cytosine-5-)-Methyltransferase 3 Like (TRDMT1))

Autre désignation: DNMT3L

Sujet: Background: DNMT 3L is a nuclear protein that has similarity to DNA methyltransferases, involved in de novo methylation of CpG islands. CpG methylation is an epigenetic modification that is important for embryonic development, imprinting, and X-chromosome inactivation. Studies in mice have demonstrated that DNA methylation is required for mammalian development. This gene encodes a nuclear protein with similarity to DNA methyltransferases. This protein is not thought to function as a DNA methyltransferase as it does not contain the amino acid residues necessary for methyltransferase activity. However, this protein does stimulate de novo methylation by DNA cytosine methyltransferase 3 alpha and it is thought to be required for the establishment of maternal genomic imprints. This protein also mediates transcriptional repression through interaction with histone deacetylase 1.

ID gène: 29947

NCBI Accession: NP_787063

UniProt: Q9UJW3