CDC16 anticorps (pThr580)

N° du produit ABIN129649

N° du produit (Fournisseur): 600-401-876

Cet anticorps Lapin Polyclonal détecte spécifiquement CDC16 dans WB, ELISA et FM. Il présente une réactivité envers Humain.

Aperçu rapide pour CDC16 anticorps (pThr580) (ABIN129649)

Antigène: CDC16 (Cell Division Cycle 16 Homolog (S. Cerevisiae) (CDC16))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp CDC16 est non-conjugé

Application: Western Blotting (WB), ELISA, Fluorescence Microscopy (FM)

Détail du produit anti-CDC16 anticorps

Épitope: pThr580

N° du produit (Fournisseur): 600-401-876

Fournisseur: Rockland

Fonction: APC6 phospho T580 Antibody

Réactivité croisée (Details): This antibody is specific for phosphorylated human APC6 protein at the pT580 residue.

Attributs du produit: Synonyms: rabbit anti-APC6 pT580 Antibody, APC 6, APC-6, Anaphase promoting complex subunit 6 antibody, CDC16Hs antibody, Cell division cycle 16 homolog antibody, Cell division cycle protein 16 homolog antibody, Cyclosome subunit 6 antibody

Purification: This product is an affinity purified antibody produced by immunoaffinity chromatography using phospho peptide coupled to agarose beads followed by solid phase adsorption(s) against non-phospho peptide and non-specific peptide to remove any unwanted reactivities.

Stérilité: Sterile filtered

Immunogène:

Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region surrounding pT580 of Human Apc6 protein.

Immunogen Type: Conjugated Peptide

Isotype: IgG

Information d'application

Indications d'application:

Application Note: This affinity purified antibody has been tested for use in ELISA and western blot.   Specific conditions for reactivity should be optimized by the end user. Expect a band ~ 72 kDa, 71.5 kDa or 65.9 kDa in size corresponding to isoforms of APC6 by western blotting in the appropriate cell lysate or extract.

Western Blot Dilution: 1:500 - 1:3,000

ELISA Dilution: 1:1,000 - 1:10,000

IF Microscopy Dilution: User Optimized

Other: User Optimized

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.97 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Date de péremption: 12 months

Détails sur CDC16

Antigène: CDC16 (Cell Division Cycle 16 Homolog (S. Cerevisiae) (CDC16))

Autre désignation: CDC16

Sujet: Background: APC6 (also known as Cell division cycle protein 16 homolog (CDC16Hs), anaphase promoting complex subunit 6, and cyclosome subunit 6) is a component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated ubiquitin ligase that controls progression through mitosis and the G₁ phase of the cell cycle.   The APC/C is composed of at least 11 subunits.   APC6 interacts with PPP5C and CDC20. This protein colocalizes with CDC27 to the centrosome at all stages of the cell cycle and to the mitotic spindle.  Splice variants have been described for this protein.

ID gène: 8881, 37537763

UniProt: Q13042

Pathways: Regulation of Actin Filament Polymerization