APC1 anticorps (pSer377)

N° du produit ABIN129650

N° du produit (Fournisseur): 600-401-877

L’anticorps Lapin Polyclonal anti-APC1 a été validé pour ELISA, IHC et WB. Il convient pour détecter APC1 dans des échantillons de Humain.

Aperçu rapide pour APC1 anticorps (pSer377) (ABIN129650)

Antigène: APC1 (ANAPC1) (Anaphase Promoting Complex Subunit 1 (ANAPC1))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp APC1 est non-conjugé

Application: ELISA, Immunohistochemistry (IHC), Western Blotting (WB)

Détail du produit anti-APC1 anticorps

Épitope: pSer377

N° du produit (Fournisseur): 600-401-877

Fournisseur: Rockland

Fonction: APC1 phospho S377 Antibody

Réactivité croisée (Details): This antibody is specific for phosphorylated human APC1 protein at the pS377 residue.

Attributs du produit: Synonyms: rabbit anti-APC1 pS377 Antibody, APC-1, APC 1, ANAPC1 antibody, Anaphase promoting complex subunit 1 antibody, Cyclosome subunit 1 antibody, MCPR antibody, Meiotic checkpoint regulator antibody

Purification: This product is an affinity purified antibody produced by immunoaffinity chromatography using phospho peptide coupled to agarose beads followed by solid phase adsorption(s) against non-phospho peptide and non-specific peptide to remove any unwanted reactivities.

Stérilité: Sterile filtered

Immunogène:

Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region surrounding pS377 of Human Apc1 protein.

Immunogen Type: Conjugated Peptide

Isotype: IgG

Information d'application

Indications d'application:

Immunohistochemistry Dilution: 5.0 μg/mL

Application Note: This affinity purified antibody has been tested for use in ELISA, immunohistochemistry and western blot.   Specific conditions for reactivity should be optimized by the end user.  Expect a band ~ 215 kDa in size corresponding to APC1 by western blotting in the appropriate cell lysate or extract.

Western Blot Dilution: 1:200 - 1:1,000

ELISA Dilution: 1:2,000 - 1:10,000

Other: User Optimized

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.21 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Date de péremption: 12 months

Détails sur APC1

Antigène: APC1 (ANAPC1) (Anaphase Promoting Complex Subunit 1 (ANAPC1))

Autre désignation: ANAPC1

Sujet: Background: APC1 (also known as Anaphase promoting complex subunit 1, Cyclosome subunit 1, Protein Tsg24, Mitotic checkpoint regulator and ANAPC1) is 1 of at least 11 subunits of the anaphase-promoting complex (APC), which functions at the metaphase-to-anaphase transition of the cell cycle and is regulated by spindle checkpoint proteins. The APC is an E3 ubiquitin ligase that targets cell cycle regulatory proteins for degradation by the proteasome, thereby allowing progression through the cell cycle.

ID gène: 64682, 12056971

UniProt: Q9H1A4