NOXO1 anticorps (AA 238-252)

N° du produit ABIN129665

N° du produit (Fournisseur): 600-401-899

L’anticorps Lapin Polyclonal anti-NOXO1 a été validé pour WB, ELISA et IHC. Il convient pour détecter NOXO1 dans des échantillons de Humain.

Aperçu rapide pour NOXO1 anticorps (AA 238-252) (ABIN129665)

Antigène: NOXO1 (NADPH Oxidase Organizer 1 (NOXO1))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp NOXO1 est non-conjugé

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Détail du produit anti-NOXO1 anticorps

Épitope: AA 238-252

N° du produit (Fournisseur): 600-401-899

Fournisseur: Rockland

Fonction: NOXO1 Antibody

Réactivité croisée (Details): Anti-NOXO-1 antibody is directed against human NOXO1 protein.

Attributs du produit: Synonyms: rabbit anti-NOXO1 antibody, NOXO-1, NOXO 1, NADPH oxidase organizer 1 antibody, NADPH oxidase regulatory protein antibody, Nox organizer 1 antibody, Nox organizing protein 1 antibody, Regulatory protein P41NOX antibody, SH3 and PX domain-containing protein 5, P41NOX, SH3PXD5

Purification: The product was affinity purified from monospecific antiserum by immunoaffinity purification.

Stérilité: Sterile filtered

Immunogène:

Immunogen: NOXO1 affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids 238-252 of human NOXO1 protein.

Immunogen Type: Conjugated Peptide

Isotype: IgG

Information d'application

Indications d'application:

Immunohistochemistry Dilution: 5-10 μg/mL

Application Note: Anti-NOXO1 antibody has been tested for use in ELISA, immunohistochemistry and western blot.  Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 50 kDa in size corresponding to NOXO1 protein by western blotting in the appropriate cell lysate or extract.

Western Blot Dilution: 1:1,000 - 1:10,000

ELISA Dilution: 1:20,000 - 1:100,000

Other: User Optimized

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1.17 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Date de péremption: 12 months

Détails sur NOXO1

Antigène: NOXO1 (NADPH Oxidase Organizer 1 (NOXO1))

Autre désignation: NOXO1

Sujet: Background: This antibody is designed, produced, and validated as part of a collaboration with the National Cancer Institute (NCI) and is suitable for Cancer, Immunology and Nuclear Signaling research. The enzymes NADPH oxidase (NOX) and dual oxidase (DUOX) generate ROS in a regulated manner, producing reactive oxygen in various cells and tissues in response to growth factors, cytokines and calcium signals. The oxidase consists of the catalytic subunit gp91phox (otherwise known as NOX2), together with the regulatory subunits p22phox, p47phox, p40phox, p67phox and the small GTPase RAC. The enzyme activity of gp91phox is regulated by the assembly of these regulatory subunits with gp91phox to form an active complex. In 1999, the first of the NOX homologues of gp91phox was described as NOX1. The enzyme was cloned from a colon epithelial cell complementary DNA library. When expressed in cells, NOX1 generated low amounts of ROS, but high-level ROS production by NOX1 was subsequently achieved by co-expression with novel regulatory subunits (described later). Subsequently, NOX3 and NOX4 were cloned, and the latter was shown to generate high levels of ROS when expressed in cells.  NOX organizer 1 (NOXO1) is a homologue of p47phox and has an almost identical domain organization, except that it lacks the auto-inhibitory region.  NOX activator 1 (NOXA1) is a homologue of p67phox and similarly shares the same domain organization, except that it lacks one of the two SH3 domains that are present in p67phox. Co-transfection of NOX1, NOXO1 and NOXA1 results in marked ROS generation. Similar to p47phox, NOXO1 binds to p22phox, which is required for NOX1-dependent activity. NOXA1 has a well-conserved activation domain, implying a conserved mechanism for regulating the activity of the target NOX enzyme.

ID gène: 124056, 16198473

UniProt: Q8NFA2