CTCFL anticorps (AA 1-30)

N° du produit ABIN129668

N° du produit (Fournisseur): 600-401-907

L’anticorps Lapin Polyclonal anti-CTCFL a été validé pour WB et ELISA. Il convient pour détecter CTCFL dans des échantillons de Humain. Il y a 6+ publications disponibles.

Aperçu rapide pour CTCFL anticorps (AA 1-30) (ABIN129668)

Antigène: CTCFL (CCCTC-Binding Factor (Zinc Finger Protein)-Like (CTCFL))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp CTCFL est non-conjugé

Application: Western Blotting (WB), ELISA

Détail du produit anti-CTCFL anticorps

Épitope: AA 1-30

N° du produit (Fournisseur): 600-401-907

Fournisseur: Rockland

Fonction: BORIS Antibody

Réactivité croisée (Details): This affinity-purified antibody is directed against the human BORIS protein.

Attributs du produit: Synonyms: rabbit anti-BORIS Antibody, Transcriptional repressor CTCFL, Brother of the regulator of imprinted sites antibody, CCCTC binding factor (zinc finger protein) like antibody, CCCTC-binding factor antibody, CTCF paralog antibody, CTCF-like, cancer/testis antigen 27, CT27, CTCF-T

Purification: The product was affinity purified from monospecific antiserum by immunoaffinity purification.

Stérilité: Sterile filtered

Immunogène:

Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to N-Terminal region near aa 1-30 of human BORIS protein.

Immunogen Type: Conjugated Peptide

Isotype: IgG

Information d'application

Indications d'application:

Application Note: BORIS affinity purified antibody has been tested for use in ELISA and by western blot.  Specific conditions for reactivity should be optimized by the end user. Expect a predominant band approximately 75 kDa in size corresponding to BORIS by western blotting in the appropriate cell lysate or extract.

Western Blot Dilution: 1:200 - 1:2,000

ELISA Dilution: 1:2,000 - 1:10,000

Other: User Optimized

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1.28 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store BORIS Antibody at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Date de péremption: 12 months

Références pour anticorps anti-CTCFL (ABIN129668)

Janssen, Moscona, Elchebly, Papadakis, Redpath, Wang, Rubin, van Kempen, Spatz: "BORIS/CTCFL promotes a switch from a proliferative towards an invasive phenotype in melanoma cells." dans: Cell death discovery, Vol. 6, pp. 1, (2020) (PubMed).

Rosa-Garrido, Ceballos, Alonso-Lecue, Abraira, Delgado, Gandarillas: "A cell cycle role for the epigenetic factor CTCF-L/BORIS." dans: PLoS ONE, Vol. 7, Issue 6, pp. e39371, (2012) (PubMed).

Macaluso, Caracciolo, Rizzo, Sun, Montanari, Russo, Bellipanni, Khalili, Giordano: "Integrating role of T antigen, Rb2/p130, CTCF and BORIS in mediating non-canonical endoplasmic reticulum-dependent death pathways triggered by chronic ER stress in mouse medulloblastoma." dans: Cell cycle (Georgetown, Tex.), Vol. 11, Issue 9, pp. 1841-50, (2012) (PubMed).

Fiorentino, Macaluso, Miranda, Montanari, Russo, Bagella, Giordano: "CTCF and BORIS regulate Rb2/p130 gene transcription: a novel mechanism and a new paradigm for understanding the biology of lung cancer." dans: Molecular cancer research : MCR, Vol. 9, Issue 2, pp. 225-33, (2011) (PubMed).

Hines, Bazarov, Mukhopadhyay, Yaswen: "BORIS (CTCFL) is not expressed in most human breast cell lines and high grade breast carcinomas." dans: PLoS ONE, Vol. 5, Issue 3, pp. e9738, (2010) (PubMed).

Dougherty, Ichim, Liu, Reznik, Min, Ghochikyan, Agadjanyan, Reznik: "Selective apoptosis of breast cancer cells by siRNA targeting of BORIS." dans: Biochemical and biophysical research communications, Vol. 370, Issue 1, pp. 109-12, (2008) (PubMed).

Détails sur CTCFL

Antigène: CTCFL (CCCTC-Binding Factor (Zinc Finger Protein)-Like (CTCFL))

Autre désignation: CTCFL

Sujet: Background: Anti-BORIS antibody is designed, produced, and validated as part of a collaboration with the National Cancer Institute (NCI) and is suitable for Cancer, Immunology and Nuclear Signaling research. BORIS (Brother of the Regulator of Imprinted Sites) also known as  CCCTC-binding factor-like protein, is  normally only expressed in the testis and expressed in a mutually exclusive manner with CTCF during male germ cell development.  However, previous studies have shown that BORIS is abnormally activated in a wide range of human cancers.  Expression of BORIS in normally BORIS-negative cells promotes cell growth that may lead to transformation.  BORIS maps to the cancer-associated amplification region thought to contain an oncogene or dominant-immortalizing gene. BORIS is a candidate protein for the epigenetic reprogramming factor acting in the male germ line.  BORIS is found in both the nucleus and cytoplasm. BORIS antibody can be used to investigate epigenetic regulation.

ID gène: 140690, 20805280

UniProt: Q8NI51