GPX4 anticorps (C-Term)

N° du produit ABIN129715

N° du produit (Fournisseur): 600-401-972

Cet anticorps Lapin Polyclonal détecte spécifiquement GPX4 dans WB, IHC et ELISA. Il présente une réactivité envers Souris, Rat et Cobaye.

Aperçu rapide pour GPX4 anticorps (C-Term) (ABIN129715)

Antigène: GPX4 (Glutathione Peroxidase 4 (GPX4))

Reactivité: Souris, Rat, Cobaye

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp GPX4 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Détail du produit anti-GPX4 anticorps

Épitope: C-Term

N° du produit (Fournisseur): 600-401-972

Fournisseur: Rockland

Fonction: Glutathione Peroxidase 4 Antibody

Réactivité croisée (Details): This affinity purified antibody is directed against mouse GPx4 protein.

Attributs du produit: Synonyms: rabbit anti-Gpx4 antibody, rabbit anti-glutathione peroxidase 4/Gpx4 antibody, rabbit anti-glutathione peroxidase 4 antibody, PHGPx antibody, Phospholipid hydroperoxide glutathione peroxidase antibody, Gpx-4, Gpx 4

Purification: The product was affinity purified from monospecific antiserum by immunoaffinity chromatography.

Stérilité: Sterile filtered

Immunogène:

Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids near the carboxyl terminus of mouse GPx4 protein.

Immunogen Type: Conjugated Peptide

Isotype: IgG

Information d'application

Indications d'application:

Immunohistochemistry Dilution: User Optimized

Application Note: This affinity purified antibody has been tested for use in ELISA and western blotting. Specific conditions for reactivity should be optimized by the end user.  Expect a band approximately 19 kDa in size corresponding to GPx4 protein by western blotting in the appropriate cell lysate or extract.

Western Blot Dilution: 1:500 - 1:3,000

ELISA Dilution: 1:7,000 - 1:30,000

Other: User Optimized

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1.15 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Date de péremption: 12 months

Détails sur GPX4

Antigène: GPX4 (Glutathione Peroxidase 4 (GPX4))

Autre désignation: Gpx4

Sujet: Background: This antibody is designed, produced, and validated as part of a collaboration with the National Cancer Institute (NCI) and is suitable for Cancer, Immunology and Nuclear Signaling research. Glutathione Peroxidase 4 (GPx4, also known as PHGPx) functions as an antioxidant and also plays a role in redox regulation, sexual maturation, inflammation, apoptosis, and differentiation.  GPx4 was found to have an important role in preventing lipid peroxidation, and has been discussed primarily as the glutathione peroxidase protecting biomembranes against oxidative stress.  GPx4 can react with free hydrogen peroxide as well as with a wide range of lipid hydroperoxides, including those derived from cholesterol and cholesteryl esters.  GPx4 is the only GPx to use phospholipid hydroperoxides as substrates.  It can also reduce thymine hydroperoxide. In contrast to GPx1 and GPx3, GPx4 can use a wide range of reducing substrates in addition to glutathione.  GPx4 is expressed in high levels in testes, suggesting a role in male fertility.  It has also been identified as an enzymatically inactive structural protein of the mitochondrial capsule of sperm.

ID gène: 625249, 13508533

UniProt: O70325