RFP anticorps (HRP)

N° du produit ABIN129725

N° du produit (Fournisseur): 600-403-379

L’anticorps Lapin Polyclonal anti-RFP a été validé pour WB, ELISA et IHC. Il convient pour détecter RFP dans des échantillons de Discosoma. Il y a 1 publication disponible.

Aperçu rapide pour RFP anticorps (HRP) (ABIN129725)

Antigène: RFP (Red Fluorescent Protein (RFP))

Reactivité: Discosoma

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp RFP est conjugé à/à la HRP

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Détail du produit anti-RFP anticorps (HRP)

N° du produit (Fournisseur): 600-403-379

Fournisseur: Rockland

Fonction: RFP Antibody Peroxidase Conjugated Pre-Adsorbed

Réactivité croisée (Details): Minimal crossreactivity against Hu Ms & Rt Serum Proteins
Expect reactivity against RFP and its variants: mCherry, tdTomato, mBanana, mOrange, mPlum, mOrange and mStrawberry.

Attributs du produit: Synonyms: rabbit anti-RFP antibody peroxidase conjugation, rabbit anti-Red Fluorescent Protein antibody peroxidase conjugation, HRP conjugated rabbit anti-RFP antibody, DsRed, rDsRed, Discosoma sp. Red Fluorescent Protein, Red fluorescent protein drFP583

Purification:

Rabbit Anti-RFP Peroxidase Conjugated Antibody was prepared from monospecific rabbit antiserum by immunoaffinity chromatography using Red Fluorescent Protein (Discosoma) coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.

Preadsorption: Pre-adsorbed

Immunogène:

Immunogen: The immunogen is a Red Fluorescent Protein (RFP) fusion protein corresponding to the full length amino acid sequence (234aa) derived from the mushroom polyp coral Discosoma.

Immunogen Type: Recombinant Protein

Isotype: IgG

Information d'application

Indications d'application:

Immunohistochemistry Dilution: 1:500 - 1:2,000

Application Note: Rabbit Anti-RFP Peroxidase Conjugated Antibody has been tested in ELISA and Western blot. It can be used to detect RFP by ELISA (sandwich or capture) for the direct binding of antigen or to detect RFP or RFP containing proteins on Western blots. References suggest that the antibody is also suitable for IHC. Optimal titers for applications should be determined by the researcher.

Western Blot Dilution: 1:2,000 - 1:5,000

ELISA Dilution: 1:10,000 - 1:50,000

Other: User Optimized

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!

Agent conservateur: Gentamicin sulfate

Précaution d'utilisation: This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 

Date de péremption: 12 months

Références pour anticorps anti-RFP (HRP) (ABIN129725)

Bohlen, El-Nahal, Sommer: "Transduction of Craniofacial Motoneurons Following Intramuscular Injections of Canine Adenovirus Type-2 (CAV-2) in Rhesus Macaques." dans: Frontiers in neuroanatomy, Vol. 13, pp. 84, (2019) (PubMed).

Détails sur RFP

Antigène: RFP (Red Fluorescent Protein (RFP))

Autre désignation: DsRed

Sujet: Background: Fluorescent proteins such as Discosoma Red Fluorescent Protein (DsRed) from sea anemone Discosoma sp. mushroom or green fluorescent protein (GFP) from Aequorea victoria jellyfish are widely used in research practice. Fusion RFP and GFP commonly serve as marker for gene expression and protein localization. As DsRed and GFP share only 19 % identity, therefore, in general, anti-GFP antibodies do not recognize DsRed protein and vice versa. Structurally, Discosoma red fluorescent protein is similar to Aequorea green fluorescent protein in terms of its overall fold (a β-can) and chromophore-formation chemistry. However, Discosoma red fluorescent protein undergoes an additional step in the chromophore maturation and obligates tetrameric structure. Rockland offers many controls, monoclonal, and polyclonal antibodies for RFP.

UniProt: Q9U6Y8