RFP anticorps (Biotin)

N° du produit ABIN129733

N° du produit (Fournisseur): 600-406-379

Cet anticorps Lapin Polyclonal détecte spécifiquement RFP dans WB, ELISA et IHC. Il présente une réactivité envers Discosoma et a été mentionné dans 4+ publications.

Aperçu rapide pour RFP anticorps (Biotin) (ABIN129733)

Antigène: RFP (Red Fluorescent Protein (RFP))

Reactivité: Discosoma

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp RFP est conjugé à/à la Biotin

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Détail du produit anti-RFP anticorps (Biotin)

N° du produit (Fournisseur): 600-406-379

Fournisseur: Rockland

Fonction: RFP Antibody Biotin Conjugated Pre-adsorbed

Réactivité croisée (Details): Minimal crossreactivity against Hu Ms & Rt Serum Proteins
Expect reactivity against RFP and its variants: mCherry, tdTomato, mBanana, mOrange, mPlum, mOrange and mStrawberry.

Attributs du produit: Synonyms: rabbit anti-RFP antibody biotin conjugation, biotin conjugated rabbit anti-RFP antibody, DsRed, rDsRed, Discosoma sp. Red Fluorescent Protein, Red fluorescent protein drFP583

Purification: This product was prepared from monospecific antiserum by immunoaffinity chromatography using Red Fluorescent Protein (Discosoma) coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.

Immunogène:

Immunogen: The immunogen is a Red Fluorescent Protein (RFP) fusion protein corresponding to the full length amino acid sequence (234aa) derived from the mushroom polyp coral Discosoma.

Immunogen Type: Recombinant Protein

Isotype: IgG

Labeling Ratio: 10-20

Information d'application

Indications d'application:

Immunohistochemistry Dilution: 1:1,000 - 1:5,000

Application Note: Polyclonal anti-RFP is designed to detect RFP and its variants. This rabbit anti-RFP Antibody Biotin Conjugated antibody has been tested by ELISA and Western blot. This product can be used to detect RFP by ELISA (sandwich or capture) for the direct binding of antigen. Biotin conjugated polyclonal anti-RFP used in a sandwich ELISA with unconjugated anti-RFP is well suited to titrate RFP in solution. The detection antibody conjugated to biotin is subsequently reacted with streptavidin conjugated HRP (code # S000-03). Optimal titers for applications should be determined by the researcher.

Western Blot Dilution: 1:2,000 - 1:10,000

ELISA Dilution: 1:10,000 - 1:80,000

Other: User Optimized

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration: 1.1 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 

Date de péremption: 12 months

Références pour anticorps anti-RFP (Biotin) (ABIN129733)

Drost, Houtman, Cseresnyés, Niesner, Rinnenthal, Miller, Prokop, Heppner: "The Amyloid-beta rich CNS environment alters myeloid cell functionality independent of their origin." dans: Scientific reports, Vol. 10, Issue 1, pp. 7152, (2020) (PubMed).

Herndler-Brandstetter, Ishigame, Shinnakasu, Plajer, Stecher, Zhao, Lietzenmayer, Kroehling, Takumi, Kometani, Inoue, Kluger, Kaech, Kurosaki, Okada, Flavell: "KLRG1+ Effector CD8+ T Cells Lose KLRG1, Differentiate into All Memory T Cell Lineages, and Convey Enhanced Protective Immunity." dans: Immunity, Vol. 48, Issue 4, pp. 716-729.e8, (2019) (PubMed).

Amano, Densmore, Lanske: "Conditional Deletion of Indian Hedgehog in Limb Mesenchyme Results in Complete Loss of Growth Plate Formation but Allows Mature Osteoblast Differentiation." dans: Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research, Vol. 30, Issue 12, pp. 2262-72, (2016) (PubMed).

Beyer, Kelly, Miquerol: "Inducible Cx40-Cre expression in the cardiac conduction system and arterial endothelial cells." dans: Genesis (New York, N.Y. : 2000), (2010) (PubMed).

Détails sur RFP

Antigène: RFP (Red Fluorescent Protein (RFP))

Autre désignation: DsRed

Sujet: Background: Fluorescent proteins such as Discosoma Red Fluorescent Protein (DsRed) from sea anemone Discosoma sp. mushroom or green fluorescent protein (GFP) from Aequorea victoria jellyfish are widely used in research practice. Fusion RFP and GFP commonly serve as marker for gene expression and protein localization. As DsRed and GFP share only 19 % identity, therefore, in general, anti-GFP antibodies do not recognize DsRed protein and vice versa. Structurally, Discosoma red fluorescent protein is similar to Aequorea green fluorescent protein in terms of its overall fold (a β-can) and chromophore-formation chemistry. However, Discosoma red fluorescent protein undergoes an additional step in the chromophore maturation and obligates tetrameric structure. Rockland offers many controls, monoclonal, and polyclonal antibodies for RFP.

UniProt: Q9U6Y8