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Nitrotyrosine anticorps

Reactivité: Différentes espèces WB, IHC (fro), IHC (p) Hôte: Souris Monoclonal EM-30 unconjugated
N° du produit ABIN1302502
  • Antigène Voir toutes Nitrotyrosine Anticorps
    Nitrotyrosine
    Reactivité
    • 20
    • 16
    • 16
    • 1
    Différentes espèces
    Hôte
    • 25
    • 23
    • 5
    Souris
    Clonalité
    • 28
    • 25
    Monoclonal
    Conjugué
    • 21
    • 7
    • 4
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Cet anticorp Nitrotyrosine est non-conjugé
    Application
    • 51
    • 27
    • 25
    • 19
    • 18
    • 14
    • 13
    • 13
    • 10
    • 10
    • 9
    • 8
    • 4
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    Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
    Specificité
    The antibody EM-30 detects nitrotyrosine group in biological material. It can be used as a marker for peroxynitrite formation in particular tissues.
    Réactivité croisée (Details)
    Broad
    Purification
    Purified by protein-A affinity chromatography.
    Pureté
    > 95 % (by SDS-PAGE)
    Immunogène
    NO2-Tyr-CH2-Thyroglobulin
    Clone
    EM-30
    Isotype
    IgG1
    Top Product
    Discover our top product Nitrotyrosine Anticorps primaire
  • Indications d'application
    Immunohistochemistry (paraffin sections): Recommended dilution: 10 μg/mL, positive tissue: colon cancer epithelium, prostate hyperplasia.
    Immunohistochemistry (frozen sections): Recommended dilution: 10 μg/mL, positive tissue: colon cancer epithelium, prostate hyperplasia.
    Western blotting: positive control: NO2-BSA, NO2-HSA.
    Restrictions
    For Research Use only
  • Concentration
    1 mg/mL
    Buffer
    Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
    Agent conservateur
    Sodium azide
    Précaution d'utilisation
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Conseil sur la manipulation
    Do not freeze.
    Stock
    4 °C
    Stockage commentaire
    Store at 2-8°C. Do not freeze.
  • Antigène
    Nitrotyrosine
    Abstract
    Nitrotyrosine Produits
    Classe de substances
    Chemical
    Sujet
    Nitrotyrosine can be detected in proteins from a variety of tissues usually in association with pathological conditions. Reaction of nitric oxide with superoxide produces peroxynitrite, which can undergo heterolytic cleavage into nitronium and hydroxyl ions. Nitration of tyrosine residues by nitronium ion forms nitrotyrosine groups in the respective proteins. Nitrotyrosine is thus a marker for inflammation-associated tissue damage.,TyrNO2, C9H10N2O5
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