SCIMP anticorps

N° du produit ABIN1302508

Cet anticorps Souris Monoclonal détecte spécifiquement SCIMP dans IP, WB, ICC et ICFC. Il présente une réactivité avec des échantillons de Humain.Il a été cité dans 1 publication.

Aperçu rapide pour SCIMP anticorps (ABIN1302508)

Antigène: SCIMP (SLP Adaptor and CSK Interacting Membrane Protein (SCIMP))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp SCIMP est non-conjugé

Application: Immunoprecipitation (IP), Western Blotting (WB), Immunocytochemistry (ICC), Intracellular Flow Cytometry (ICFC)

Clone: NVL-07

Détail du produit anti-SCIMP anticorps

Fonction: Anti-SCIMP Purified

Réactivité croisée (Details): Human

Attributs du produit: The mouse monoclonal antibody NVL-07 recognizes intracellular part of human transmembrane adaptor SCIMP. This protein of 17 kDa predicted Mw migrates as a 22 kDa band on SDS PAGE.

Purification: Purified by protein-A affinity chromatography.

Pureté: > 95 % (by SDS-PAGE)

Immunogène: Recombinant intracellular part of human SCIMP

Isotype: IgG2a

Information d'application

Indications d'application: Flow cytometry: Recommended dilution: 1-5 μg/mL. Intracellular staining.
Western blotting: Recommended dilution: 1-2 μg/mL.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Do not freeze

Stock: 4 °C

Stockage commentaire: Store at 2-8°C. Do not freeze.

Références pour anticorps anti-SCIMP (ABIN1302508)

Draber, Vonkova, Stepanek, Hrdinka, Kucova, Skopcova, Otahal, Angelisova, Horejsi, Yeung, Weiss, Brdicka: "SCIMP, a transmembrane adaptor protein involved in major histocompatibility complex class II signaling." dans: Molecular and cellular biology, Vol. 31, Issue 22, pp. 4550-62, (2011) (PubMed).

Détails sur SCIMP

Antigène: SCIMP (SLP Adaptor and CSK Interacting Membrane Protein (SCIMP))

Autre désignation: SCIMP

Sujet: SLP adaptor and CSK interacting membrane protein,SCIMP (SLP adaptor and Csk interacting membrane protein), also known as Nvl, is a palmitoylated transmembrane adaptor protein expressed in professional antigen presenting cells, most prominently in the lymph nodes and spleen. It is associated with tetraspanin-enriched microdomains (together with MHC II). There is a close relationship between SCIMP and tyrosinkinase Lyn, which is constitutively bound to it by its SH3 domain. After MHC II-mediated stimulation in the immunological synapse SCIMP becomes phosphorylated at several tyrosine residues and provides docking sites for Grb2 and SLP65 or SLP76 adaptors transducing the signal downstream, as well as for the kinase Csk with modulatory roles.,NVL

ID gène: 388325

UniProt: Q6UWF3