EpCAM anticorps (APC)

N° du produit ABIN1302618

Cet anticorps anti-EpCAM est un anticorps Souris Monoclonal détectant EpCAM dans FACS. Adapté pour Humain. Ce Primary Antibody a été cité dans 4+ publications.

Aperçu rapide pour EpCAM anticorps (APC) (ABIN1302618)

Antigène: EpCAM (EPCAM) (Epithelial Cell Adhesion Molecule (EPCAM))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp EpCAM est conjugé à/à la APC

Application: Flow Cytometry (FACS)

Clone: VU-1D9

Détail du produit anti-EpCAM anticorps (APC)

Fonction: Anti-Hu CD326 APC

Specificité: The mouse monoclonal antibody VU-1D9 recognizes an extracellular epitope within EGF-like domain I of CD326 / EpCAM, a marker of epithelial lineages. This antibody strongly stains various normal epithelial cells and carcinomas.

Réactivité croisée (Details): Human, Other species Not tested

Purification: Purified antibody is conjugated with activated allophycocyanin (APC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

Immunogène: Small cell lung carcinoma cell line H69.

Isotype: IgG1

Information d'application

Indications d'application: Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μL reagent / 100 μL of whole blood or 10⁶ cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.

Restrictions: For Research Use only

Stockage

Buffer: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid prolonged exposure to light.
Do not use after expiration date stamped on vial label.
Short-term exposure to room temperature should not affect the quality of the reagent. However, if reagent is stored under any conditions other than those specified, the conditions must be verified by the user.

Stock: 4 °C

Stockage commentaire: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

Références pour anticorps anti-EpCAM (APC) (ABIN1302618)

Brunner, Prelog, Verdorfer, Tzankov, Mikuz, Ensinger: "EpCAM is predominantly expressed in high grade and advanced stage urothelial carcinoma of the bladder." dans: Journal of clinical pathology, Vol. 61, Issue 3, pp. 307-10, (2008) (PubMed).

Winter, Nagtegaal, van Krieken, Litvinov: "The epithelial cell adhesion molecule (Ep-CAM) as a morphoregulatory molecule is a tool in surgical pathology." dans: The American journal of pathology, Vol. 163, Issue 6, pp. 2139-48, (2003) (PubMed).

Li, Passebosc-Faure, Lambert, Gentil-Perret, Blanc, Oosterwijk, Mosnier, Genin, Tostain: "Flow cytometric analysis of antigen expression in malignant and normal renal cells." dans: Anticancer research, Vol. 20, Issue 4, pp. 2773-8, (2000) (PubMed).

Tsubura, Senzaki, Sasaki, Hilgers, Morii: "Immunohistochemical demonstration of breast-derived and/or carcinoma-associated glycoproteins in normal skin appendages and their tumors." dans: Journal of cutaneous pathology, Vol. 19, Issue 1, pp. 73-9, (1992) (PubMed).

Détails sur EpCAM

Antigène: EpCAM (EPCAM) (Epithelial Cell Adhesion Molecule (EPCAM))

Autre désignation: CD326

Sujet: Epithelial cell adhesion molecule,CD326 / EpCAM (also known as ESA, EGP40, EGP-2, KSA1/4, CO17-1A, GA733-2, MOC31, Ber-EP4) is a 40 kDa transmembrane glycoprotein serving as adhesion molecule in the basolateral membranes in a variety of epithelial cells. CD326 mediates calcium-independent homotypic cell-cell adhesions. CD326 over-expression has been detected in many epithelial tumours and is often associated with bad prognosis. It has been used for diagnostics of (pre-) malignancies at early stages.,EPCAM, GA733-2, EGP314, KSA, KS 1/4 antigen, Trop-1, M4S1, DIAR5, MIC18, TROP1

ID gène: 4072

UniProt: P16422