EpCAM anticorps (APC)

N° du produit ABIN1302618

Détail du produit anti-EpCAM anticorps (APC)

Antigène: EpCAM (EPCAM) (Epithelial Cell Adhesion Molecule (EPCAM))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp EpCAM est conjugé à/à la APC

Application: Flow Cytometry (FACS)

Specificité: The mouse monoclonal antibody VU-1D9 recognizes an extracellular epitope within EGF-like domain I of CD326 / EpCAM, a marker of epithelial lineages. This antibody strongly stains various normal epithelial cells and carcinomas.

Réactivité croisée (Details): Human, Other species Not tested

Purification: Purified antibody is conjugated with activated allophycocyanin (APC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

Immunogène: Small cell lung carcinoma cell line H69.

Clone: VU-1D9

Isotype: IgG1

Information d'application

Indications d'application: Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μL reagent / 100 μL of whole blood or 10⁶ cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.

Commentaires:

The purified antibody is conjugated with cross-linked Allophycocyanin (APC) under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use. No reconstitution is necessary.

Restrictions: For Research Use only

Stockage

Buffer: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid prolonged exposure to light.
Do not use after expiration date stamped on vial label.
Short-term exposure to room temperature should not affect the quality of the reagent. However, if reagent is stored under any conditions other than those specified, the conditions must be verified by the user.

Stock: 4 °C

Stockage commentaire: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

Références pour anticorps anti-EpCAM (APC) (ABIN1302618)

Brunner, Prelog, Verdorfer, Tzankov, Mikuz, Ensinger: "EpCAM is predominantly expressed in high grade and advanced stage urothelial carcinoma of the bladder." dans: Journal of clinical pathology, Vol. 61, Issue 3, pp. 307-10, (2008) (PubMed).

Winter, Nagtegaal, van Krieken, Litvinov: "The epithelial cell adhesion molecule (Ep-CAM) as a morphoregulatory molecule is a tool in surgical pathology." dans: The American journal of pathology, Vol. 163, Issue 6, pp. 2139-48, (2003) (PubMed).

Li, Passebosc-Faure, Lambert, Gentil-Perret, Blanc, Oosterwijk, Mosnier, Genin, Tostain: "Flow cytometric analysis of antigen expression in malignant and normal renal cells." dans: Anticancer research, Vol. 20, Issue 4, pp. 2773-8, (2000) (PubMed).

Tsubura, Senzaki, Sasaki, Hilgers, Morii: "Immunohistochemical demonstration of breast-derived and/or carcinoma-associated glycoproteins in normal skin appendages and their tumors." dans: Journal of cutaneous pathology, Vol. 19, Issue 1, pp. 73-9, (1992) (PubMed).

Détails sur EpCAM

Antigène: EpCAM (EPCAM) (Epithelial Cell Adhesion Molecule (EPCAM))

Autre désignation: CD326 / EpCAM (EPCAM Produits)

Synonymes: anticorps DIAR5, anticorps EGP-2, anticorps EGP314, anticorps EGP40, anticorps ESA, anticorps HNPCC8, anticorps KS1/4, anticorps KSA, anticorps M4S1, anticorps MIC18, anticorps MK-1, anticorps TACSTD1, anticorps TROP1, anticorps ECS-1, anticorps ECS1, anticorps ESA1, anticorps M17S1, anticorps EGP, anticorps Ep-CAM, anticorps sb:cb6, anticorps tacstd, anticorps wu:fj17g02, anticorps zgc:110304, anticorps zgc:77119, anticorps tacstd1, anticorps MGC80540, anticorps EPCAM, anticorps egp, anticorps ksa, anticorps m4s1, anticorps mk-1, anticorps cd326, anticorps egp40, anticorps mic18, anticorps trop1, anticorps ep-cam, anticorps hegp-2, anticorps co17-1a, anticorps ga733-2, anticorps CD326, anticorps Egp314, anticorps EpCAM1, anticorps GA733-2, anticorps Ly74, anticorps Tacsd1, anticorps Tacstd1, anticorps gp40, anticorps epithelial cell adhesion molecule, anticorps flotillin 2, anticorps epithelial cell adhesion molecule S homeolog, anticorps EPCAM, anticorps FLOT2, anticorps epcam, anticorps epcam.S, anticorps Epcam

Sujet: Epithelial cell adhesion molecule,CD326 / EpCAM (also known as ESA, EGP40, EGP-2, KSA1/4, CO17-1A, GA733-2, MOC31, Ber-EP4) is a 40 kDa transmembrane glycoprotein serving as adhesion molecule in the basolateral membranes in a variety of epithelial cells. CD326 mediates calcium-independent homotypic cell-cell adhesions. CD326 over-expression has been detected in many epithelial tumours and is often associated with bad prognosis. It has been used for diagnostics of (pre-) malignancies at early stages.,EPCAM, GA733-2, EGP314, KSA, KS 1/4 antigen, Trop-1, M4S1, DIAR5, MIC18, TROP1

ID gène: 4072

UniProt: P16422